PMID- 10406898
OWN - NLM
STAT- MEDLINE
DCOM- 19990824
LR  - 20151119
IS  - 0390-6078 (Print)
IS  - 0390-6078 (Linking)
VI  - 84
IP  - 7
DP  - 1999 Jul
TI  - 13q14 deletion in non-Hodgkin's lymphoma: correlation with clinicopathologic 
      features.
PG  - 589-93
AB  - BACKGROUND AND OBJECTIVE: 13q14 deletion frequently occurs as a single anomaly in 
      chronic lymphocytic leukemia (CLL) with favorable prognosis. This study was 
      performed to assess the distribution of 13q14 deletion in non-Hodgkin's lymphoma 
      (NHL) and to analyze its correlation with salient clinicopathologic features. 
      DESIGN AND METHODS: One hundred and twenty-five NHL were analyzed by cytogenetics 
      and by interphase fluorescence in situ hybridization (FISH), using a 13q14 cosmid 
      probe recognizing DNA sequences between the Rb gene and the D13S25 marker. 
      Clinical records all patients were surveyed. RESULTS: A 13q14 rearrangement was 
      present in the stemline in 10 patients; 15 additional cases were shown by FISH to 
      carry 13q14 deletion in 55-90% of the interphase cells, giving a 20% overall 
      incidence for this anomaly. Six of 44 patients had a low-grade NHL, 14/28 had 
      mantle cell lymphoma (MCL), 5/42 had a high grade NHL (p<0.0001). There was not 
      correlation between 13q, karyotype status and complexity. A statistically 
      significant association was found between 13q-, presence of splenomegaly and PB 
      involvement, lower probability of attaining complete remission (CR) and shorter 
      survival. These findings were not simply a function of the association of 13q- 
      with MCL. In multivariate analysis, a complex karyotype had prognostic importance 
      (p=0.0078), along with age (p=0.01), histology (p=0.001), LDH (p=0.03), PS 
      (p=0.001), sex (p=0.03) and splenomegaly (p=0.02). INTERPRETATION AND 
      CONCLUSIONS: 13q14 deletion represented an early chromosome change and showed a 
      preferential association with MCL, though it was found in virtually all principal 
      histologic subtypes, irrespective of clinical stage, karyotype status and 
      complexity. Patients with 13q14 deletions had a low CR rate, suggesting that 
      genes relevant to lymphomagenesis are located in this chromosome segment that 
      warrants molecular cytogenetic investigation.
FAU - Cuneo, A
AU  - Cuneo A
AD  - Istituto di Ematologia, Universita di Ferrara, via Savonarola 9, 44100 Ferrara, 
      Italy. sse@dns.unife.it
FAU - Bigoni, R
AU  - Bigoni R
FAU - Rigolin, G M
AU  - Rigolin GM
FAU - Roberti, M G
AU  - Roberti MG
FAU - Bardi, A
AU  - Bardi A
FAU - Campioni, D
AU  - Campioni D
FAU - Minotto, C
AU  - Minotto C
FAU - Agostini, P
AU  - Agostini P
FAU - Milani, R
AU  - Milani R
FAU - Bullrich, F
AU  - Bullrich F
FAU - Negrini, M
AU  - Negrini M
FAU - Croce, C
AU  - Croce C
FAU - Castoldi, G
AU  - Castoldi G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - *Biomarkers, Tumor
MH  - *Chromosomes, Human, Pair 13
MH  - Gene Rearrangement
MH  - Genes, Retinoblastoma
MH  - Humans
MH  - Karyotyping
MH  - Lymphoma, Non-Hodgkin/*genetics/physiopathology
MH  - Multivariate Analysis
MH  - Prognosis
MH  - *Sequence Deletion
EDAT- 1999/07/16 00:00
MHDA- 1999/07/16 00:01
CRDT- 1999/07/16 00:00
PHST- 1999/07/16 00:00 [pubmed]
PHST- 1999/07/16 00:01 [medline]
PHST- 1999/07/16 00:00 [entrez]
PST - ppublish
SO  - Haematologica. 1999 Jul;84(7):589-93.