PMID- 10407182
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 71
IP  - 1
DP  - 1999 Jul 23
TI  - Hippocampal BDNF mRNA shows a diurnal regulation, primarily in the exon III 
      transcript.
PG  - 11-22
AB  - Endogenous expression levels of brain-derived neurotrophic factor (BDNF) mRNA 
      were assessed using in situ hybridization to investigate whether there is a 
      natural diurnal fluctuation in BDNF mRNA expression in the hippocampus of rats 
      housed with a normal (12:12 h) light/dark cycle. BDNF expression was increased 
      during lights out (dark-cycle) to 134%-158% of light-cycle levels in hippocampal 
      regions CA1, CA3, and hilus. In addition, expression levels of the four BDNF 
      transcript forms, exons I-IV, were assessed to evaluate whether expression of 
      specific BDNF transcripts exhibited differential endogenous fluctuation. All 
      exons had lowest levels of expression at either noon or 6 p.m. Significant 
      correlations were found between exon expression level and time, with elevated 
      expression occurring at dark-cycle timepoints. The exon III transcript showed the 
      greatest diurnal change in expression in all hippocampal fields, with dark-cycle 
      expression elevated to 219-419% of light-cycle expression level. In addition to 
      exon III, dark-cycle exon II mRNA levels were elevated in all hippocampal 
      subfields, to 140-180% of light-cycle levels, suggesting that the endogenous 
      fluctuation in BDNF expression results predominantly from activation of the 
      promoters linked to exons II and III. Previously we have shown that physical 
      activity increases BDNF expression. The naturally occurring rise in BDNF 
      expression during the dark-cycle, the time when rats are most physically active, 
      may be due to increased activity and arousal levels. Because BDNF has a role in 
      plasticity, the increase in BDNF expression during the time that a rat is 
      maximally interacting with its surroundings may be part of an ongoing 
      stimulus-encoding mechanism, or may be a mechanism to maximize information 
      storage about the environment.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Berchtold, N C
AU  - Berchtold NC
AD  - Institute for Brain Aging and Dementia, University of California, Irvine, 1226 
      Gillespie Building, Irvine, CA 92697-4540, USA. nberchto@uci.edu
FAU - Oliff, H S
AU  - Oliff HS
FAU - Isackson, P
AU  - Isackson P
FAU - Cotman, C W
AU  - Cotman CW
LA  - eng
GR  - AG 13411/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - *Circadian Rhythm
MH  - Darkness
MH  - Dentate Gyrus/metabolism
MH  - *Exons
MH  - *Gene Expression Regulation
MH  - Hippocampus/*metabolism
MH  - In Situ Hybridization
MH  - Light
MH  - Male
MH  - Neuronal Plasticity
MH  - Neurons/metabolism
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Inbred F344
MH  - *Transcription, Genetic
EDAT- 1999/07/17 00:00
MHDA- 1999/07/17 00:01
CRDT- 1999/07/17 00:00
PHST- 1999/07/17 00:00 [pubmed]
PHST- 1999/07/17 00:01 [medline]
PHST- 1999/07/17 00:00 [entrez]
AID - S0169328X99001370 [pii]
AID - 10.1016/s0169-328x(99)00137-0 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Jul 23;71(1):11-22. doi: 
      10.1016/s0169-328x(99)00137-0.