PMID- 10407194
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 71
IP  - 1
DP  - 1999 Jul 23
TI  - Molecular cloning, expression and characterization of a bovine serotonin 
      transporter.
PG  - 120-6
AB  - The serotonin transporter (SERT) is a member of a highly homologous family of 
      sodium/chloride dependent neurotransmitter transporters responsible for reuptake 
      of biogenic amines from the extracellular fluid. SERT constitutes the 
      pharmacological target of several clinically important antidepressants. Here we 
      report the molecular cloning of SERT from the bovine species. Translation of the 
      nucleotide sequence revealed 44 amino acid differences compared to human SERT. 
      When transiently expressed in HeLa cells and compared with rat and human SERTs 
      the K(m) value for uptake was increased 2-fold. V(max) and B(max) were both 
      increased about 4-fold indicating the turnover number is conserved. The 
      pharmacological profile revealed a decreased sensitivity towards imipramine, 
      desipramine, citalopram, fluoxetine and paroxetine compared with human SERT, 
      while the sensitivity towards 3, 4-methylenedioxymethamphetamine (MDMA) was 
      mainly unchanged. RT-PCR amplification of RNA from different tissues demonstrated 
      expression of SERT in placenta, brain stem, bone marrow, kidney, lung, heart, 
      adrenal gland, liver, parathyroid gland, thyroid gland, small intestine and 
      pancreas.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Mortensen, O V
AU  - Mortensen OV
AD  - Department of Biological Psychiatry, Psychiatric University Hospital, Skovagervej 
      2, 8240 Risskov, Denmark.
FAU - Kristensen, A S
AU  - Kristensen AS
FAU - Rudnick, G
AU  - Rudnick G
FAU - Wiborg, O
AU  - Wiborg O
LA  - eng
SI  - GENBANK/AF119122
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SLC6A4 protein, human)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 0 (Slc6a4 protein, rat)
RN  - 01K63SUP8D (Fluoxetine)
RN  - 0DHU5B8D6V (Citalopram)
RN  - 333DO1RDJY (Serotonin)
RN  - 41VRH5220H (Paroxetine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - OGG85SX4E4 (Imipramine)
RN  - TG537D343B (Desipramine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/*genetics/metabolism/*physiology
MH  - Cattle
MH  - Citalopram/pharmacology
MH  - Cloning, Molecular
MH  - Desipramine/pharmacology
MH  - Female
MH  - Fluoxetine/pharmacology
MH  - HeLa Cells
MH  - Humans
MH  - Imipramine/pharmacology
MH  - Kinetics
MH  - Membrane Glycoproteins/*genetics/metabolism/*physiology
MH  - *Membrane Transport Proteins
MH  - Molecular Sequence Data
MH  - N-Methyl-3,4-methylenedioxyamphetamine/pharmacology
MH  - *Nerve Tissue Proteins
MH  - Organ Specificity
MH  - Paroxetine/pharmacology
MH  - Phylogeny
MH  - Pregnancy
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Serotonin/metabolism
MH  - Serotonin Plasma Membrane Transport Proteins
MH  - Transfection
EDAT- 1999/07/17 00:00
MHDA- 1999/07/17 00:01
CRDT- 1999/07/17 00:00
PHST- 1999/07/17 00:00 [pubmed]
PHST- 1999/07/17 00:01 [medline]
PHST- 1999/07/17 00:00 [entrez]
AID - S0169328X99001783 [pii]
AID - 10.1016/s0169-328x(99)00178-3 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Jul 23;71(1):120-6. doi: 
      10.1016/s0169-328x(99)00178-3.