PMID- 10407498
OWN - NLM
STAT- MEDLINE
DCOM- 19990916
LR  - 20190718
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 144
IP  - 2
DP  - 1999 Jun
TI  - Monokine stimulation of interleukin-11 production by human vascular smooth muscle 
      cells in vitro.
PG  - 375-80
AB  - Human vascular smooth muscle cells (VSMC) are a component of blood vessels, and 
      secrete a variety of cytokines in atherosclerotic loci. Interleukin-11 (IL-11), a 
      member of IL-6-like cytokines, is reported to be involved in inflammation and 
      tissue remodeling, both of which are observed in atherosclerosis. However, no 
      information is available as to the production of IL-11 by VSMC. Therefore, the 
      expression of IL-11 in VSMC is investigated. The amounts of IL-11 protein and 
      mRNA were determined by enzyme-linked immunosorbent assay (ELISA) and Northern 
      blot analysis, respectively. The expression of IL-11 in VSMC was also 
      immunohistochemically determined. IL-1 alpha, transforming growth factor-beta 
      (TGF beta) and, to a lesser extent, tumor necrosis factor-alpha (TNF alpha) 
      stimulated the IL-11 production by VSMC, and the stimulatory effects of IL-1 
      alpha and TGF beta on IL-11 production were dose-dependent. IL-1 alpha and TNF 
      alpha synergistically augmented TGF beta-stimulated IL-11 production by VSMC. 
      Immunohistochemical staining also revealed the expression of IL-11 protein in 
      VSMC. Furthermore, IL-1 alpha, TGF beta, and TNF alpha induced IL-11 gene 
      expression in VSMC. Because IL-6-like cytokines are reported to be 
      cytoprotective, monokine-stimulated IL-11 may have a potent protective role in 
      atherosclerotic lesions.
FAU - Taki, H
AU  - Taki H
AD  - First Department of Internal Medicine, Toyama Medical and Pharmaceutical 
      University, Japan. htaki-tym@umin.ac.jp
FAU - Sakai, T
AU  - Sakai T
FAU - Sugiyama, E
AU  - Sugiyama E
FAU - Mino, T
AU  - Mino T
FAU - Kuroda, A
AU  - Kuroda A
FAU - Taki, K
AU  - Taki K
FAU - Hamazaki, T
AU  - Hamazaki T
FAU - Koizumi, H
AU  - Koizumi H
FAU - Kobayashi, M
AU  - Kobayashi M
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-11)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Monokines)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Arteriosclerosis/immunology
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Interleukin-11/genetics/*metabolism
MH  - Lymphotoxin-alpha/pharmacology
MH  - Monokines/*pharmacology
MH  - Muscle, Smooth, Vascular/*immunology
MH  - Recombinant Proteins/pharmacology
MH  - Stimulation, Chemical
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1999/07/17 00:00
MHDA- 1999/07/17 00:01
CRDT- 1999/07/17 00:00
PHST- 1999/07/17 00:00 [pubmed]
PHST- 1999/07/17 00:01 [medline]
PHST- 1999/07/17 00:00 [entrez]
AID - S0021-9150(99)00009-X [pii]
AID - 10.1016/s0021-9150(99)00009-x [doi]
PST - ppublish
SO  - Atherosclerosis. 1999 Jun;144(2):375-80. doi: 10.1016/s0021-9150(99)00009-x.