PMID- 10408098
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20061115
IS  - 0008-4166 (Print)
IS  - 0008-4166 (Linking)
VI  - 45
IP  - 3
DP  - 1999 Mar
TI  - Staphylococcus aureus isogenic mutant, deficient in toxic shock syndrome toxin-1 
      but not staphylococcal enterotoxin A production, exhibits attenuated virulence in 
      a tampon-associated vaginal infection model of toxic shock syndrome.
PG  - 250-6
AB  - Since menstrual toxic shock syndrome (MTSS) is associated with a predominant 
      clone of Staphylococcus aureus which produces both toxic shock syndrome toxin-1 
      (TSST-1) and staphylococcal enterotoxin A (SEA), we sought to clarify the role of 
      TSST-1 in a tampon-associated vaginal infection model in New Zealand White (NZW) 
      rabbits, using isogenic tst+/sea+ S. aureus mutants in which tst was inactivated 
      by allelic replacement. Rabbits infected with the tst-/sea+ strain became ill 
      within 3 days, with fever, weight loss, conjunctival hyperemia, and lethargy. 
      Mortality was significantly higher with the tst+/sea+ strain compared to its 
      tst-/sea+ isogenic derivative (4/13 vs. 0/14; p < 0.05, Fisher's exact test, 
      2-tailed). Mean fever index was higher (p < 0.005; t test, 2-tailed) and weight 
      loss more sustained among survivors in the tst+/sea+ group. Furthermore, culture 
      filtrates from the tst+/sea+ strain induced a significantly greater response in 
      mitogenesis and TNF alpha secretion from rabbit splenocytes in vitro compared to 
      the tst-/sea+ isogenic derivative. Thus, regardless of the role of SEA, TSST-1 
      significantly contributed to both morbidity and mortality in this 
      tampon-associated vaginal infection model in NZW rabbits. This is the first 
      demonstration of the potential role of TSST-1 and SEA in the pathogenesis of MTSS 
      with a MTSS-associated clinical S. aureus strain in a relevant animal model.
FAU - De Boer, M L
AU  - De Boer ML
AD  - Department of Medicine, University of British Columbia, Vancouver, Canada.
FAU - Kum, W W
AU  - Kum WW
FAU - Chow, A W
AU  - Chow AW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Microbiol
JT  - Canadian journal of microbiology
JID - 0372707
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Superantigens)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (enterotoxin F, Staphylococcal)
RN  - 37337-57-8 (enterotoxin A, Staphylococcal)
SB  - IM
MH  - Animals
MH  - *Bacterial Toxins
MH  - *Disease Models, Animal
MH  - Enterotoxins/analysis/*genetics/metabolism/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoblotting
MH  - Mutation
MH  - Rabbits
MH  - Shock, Septic/immunology/*microbiology/mortality
MH  - Staphylococcal Infections/immunology/*microbiology/mortality
MH  - *Staphylococcus aureus/chemistry/genetics/pathogenicity
MH  - *Superantigens
MH  - Tumor Necrosis Factor-alpha/analysis
MH  - Virulence
EDAT- 1999/07/17 00:00
MHDA- 1999/07/17 00:01
CRDT- 1999/07/17 00:00
PHST- 1999/07/17 00:00 [pubmed]
PHST- 1999/07/17 00:01 [medline]
PHST- 1999/07/17 00:00 [entrez]
PST - ppublish
SO  - Can J Microbiol. 1999 Mar;45(3):250-6.