PMID- 10408793
OWN - NLM
STAT- MEDLINE
DCOM- 19990812
LR  - 20191103
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 8
IP  - 1
DP  - 1999 Mar
TI  - Cytogenetic aberrations in myelodysplastic syndrome detected by comparative 
      genomic hybridization and fluorescence in situ hybridization.
PG  - 47-53
AB  - Conventional cytogenetics (CC) is proven as a diagnostic and prognostic factor in 
      myelodysplastic syndrome (MDS). However, CC may be hampered by insufficient 
      metaphase preparation and cannot analyze interphase nuclei. These problems are 
      solved by using comparative genomic hybridization (CGH). The CGH was applied to 
      samples from 45 patients with MDS, and the results were compared with CC and 
      fluorescence in situ hybridization (FISH). The CC detected aberrations in 12 of 
      45 samples, including chromosomes 3 (n = 1), 5 (n = 9), 7 (n = 2),8(n = 1), 18(n 
      = 1),21 (n = 1), X (n = 1), and Y(n = 2). In one patient, loss of B and C group 
      chromosomes and a marker chromosome were seen. The CGH revealed chromosomal 
      imbalances in 18 of 45 samples, including chromosomes 5 (n = 11), 7 (n = 2), 8 (n 
      = 1), 18(n = 1), 20(n = 1), 21 (n = 1), X (n = 1), and Y (n = 2). All unbalanced 
      aberrations found by CC were detected by CGH, too. In two patients, the CGH found 
      additional aberrations and redefined the aberrations of the chromosomes of the B 
      and C group in one sample. The FISH confirmed these aberrations. Additionally 
      performed FISH for chromosomes 5, 7, and 8 gave normal findings in all patients 
      found to be normal in CC and CGH. The CGH and FISH confirmed the results obtained 
      by CC. All three techniques showed changes of chromosomes 5 and 7 as the most 
      frequent aberrations, emphasizing the importance of these chromosomes in the 
      development of MDS. Furthermore, the CC is proven as the basic technique for 
      cytogenetic evaluation of MDS.
FAU - Wilkens, L
AU  - Wilkens L
AD  - Pathologisches Institut, der Medizinischen Hochschule Hannover, Germany.
FAU - Burkhardt, D
AU  - Burkhardt D
FAU - Tchinda, J
AU  - Tchinda J
FAU - Busche, G
AU  - Busche G
FAU - Werner, M
AU  - Werner M
FAU - Nolte, M
AU  - Nolte M
FAU - Ganser, A
AU  - Ganser A
FAU - Georgii, A
AU  - Georgii A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow Cells/pathology
MH  - Child
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 5
MH  - DNA/analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukocytes/cytology
MH  - Male
MH  - Metaphase
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics/pathology
MH  - Nucleic Acid Hybridization/*methods
EDAT- 1999/07/17 00:00
MHDA- 1999/07/17 00:01
CRDT- 1999/07/17 00:00
PHST- 1999/07/17 00:00 [pubmed]
PHST- 1999/07/17 00:01 [medline]
PHST- 1999/07/17 00:00 [entrez]
AID - 10.1097/00019606-199903000-00008 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 1999 Mar;8(1):47-53. doi: 10.1097/00019606-199903000-00008.