PMID- 10408862
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20181113
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 80
IP  - 3-4
DP  - 1999 May
TI  - c-erbB2/neu gene and chromosome 17 analysis in breast cancer by FISH on archival 
      cytological fine-needle aspirates.
PG  - 519-25
AB  - The detection of specific genetic alterations in breast cancer is useful for 
      diagnosing, predicting prognosis and planning preoperative treatment. c-erbB2/neu 
      overexpression is usually detected by immunocytochemistry (ICC), although this 
      technique is neither completely reproducible nor highly reliable, owing to 
      specimen and methodologic variability and antibody sensitivity. Here, we combine 
      two well-established techniques, fine-needle aspiration (FNA) and fluorescence in 
      situ hybridization (FISH), to detect c-erbB2/neu amplification in patients 
      candidate to primary chemotherapy and, in part, previously analysed for 
      c-erbB2/neu overexpression. Sixty smears from FNA were used to simultaneously 
      detect c-erbB2/neu and chromosome 17 centromere. FISH was successful in 58 cases 
      and detected 24 amplified cases, three of which were negative by 
      immunophenotyping, 28 negative cases, with evidence of two normal 
      c-erbB2/neu/signals, two cases with deletion of c-erbB2/neu, and four cases with 
      polysomy, thus providing more reliable and informative results than ICC. This 
      study underlines the advantages offered by the FNA and FISH combination which are 
      two rapid, reliable, simple and informative techniques, to analyse one of the 
      most important genetic markers for predicting prognosis and chemotherapy planning 
      for breast carcinoma in particular in the light of the recently proposed trials 
      of primary chemotherapy.
FAU - Mezzelani, A
AU  - Mezzelani A
AD  - Divisione di Anatomia e Istologia Patologica e Citopatologia, Istituto Nazional e 
      per lo Studio e la Cura dei Tumouri, Milano, Italy.
FAU - Alasio, L
AU  - Alasio L
FAU - Bartoli, C
AU  - Bartoli C
FAU - Bonora, M G
AU  - Bonora MG
FAU - Pierotti, M A
AU  - Pierotti MA
FAU - Rilke, F
AU  - Rilke F
FAU - Pilotti, S
AU  - Pilotti S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - Biopsy, Needle/*methods
MH  - Breast Neoplasms/*genetics/*pathology
MH  - *Chromosomes, Human, Pair 17
MH  - Female
MH  - Gene Amplification
MH  - Gene Deletion
MH  - *Genes, erbB-2
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Middle Aged
PMC - PMC2362342
EDAT- 1999/07/17 00:00
MHDA- 1999/07/17 00:01
PMCR- 2000/05/01
CRDT- 1999/07/17 00:00
PHST- 1999/07/17 00:00 [pubmed]
PHST- 1999/07/17 00:01 [medline]
PHST- 1999/07/17 00:00 [entrez]
PHST- 2000/05/01 00:00 [pmc-release]
AID - 6690387 [pii]
AID - 10.1038/sj.bjc.6690387 [doi]
PST - ppublish
SO  - Br J Cancer. 1999 May;80(3-4):519-25. doi: 10.1038/sj.bjc.6690387.