PMID- 10410918
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20191103
IS  - 0905-6157 (Print)
IS  - 0905-6157 (Linking)
VI  - 10
IP  - 1
DP  - 1999 Feb
TI  - In vivo histamine release during the first minutes after deliberate sting 
      challenges correlates with the severity of allergic symptoms.
PG  - 53-7
AB  - In this study, deliberate sting challenge was investigated as a method for 
      estimating the severity of anaphylactic reactions in bee venom-sensitized 
      subjects. Twenty-one patients with previous anaphylactic reactions to field bee 
      sting were subjected to a deliberate sting challenge (n = 32). To document 
      anaphylactic reactions, plasma histamine levels were measured before, and then 1 
      and 2 min after, bee sting challenge. Eleven patients were re-challenged after 
      3-5 weeks. On 18 occasions, sting challenges caused no systemic reactions, in 
      seven cases reactions were mild, in five moderate and in two severe. In all 
      children showing systemic reactions, significant increases of plasma histamine 
      were measured after 2 min. The results correlated significantly with clinical 
      scores but not with skin prick test or with specific immunoglobulin E (IgE) and 
      immunoglobulin G (IgG) antibodies against bee venom. In patients developing local 
      reactions only, no increase of plasma histamine was detected. The relative amount 
      of released histamine correlated significantly with the severity of clinical 
      symptoms. Significant histamine release occured during the first 2 min after 
      sting challenge in children with subsequent systemic reactions and the severity 
      of these subsequent anaphylactic reactions correlated with plasma histamine 
      concentrations. The measurement of plasma histamine levels in the first minutes 
      after challenge test may therefore be used as an objective marker of a potential 
      systemic reaction.
FAU - Halmerbauer, G
AU  - Halmerbauer G
AD  - University Children's Hospital, Vienna, Austria.
FAU - Hauk, P
AU  - Hauk P
FAU - Forster, J
AU  - Forster J
FAU - Urbanek, R
AU  - Urbanek R
FAU - Kaufmehl, K
AU  - Kaufmehl K
FAU - Koller, D Y
AU  - Koller DY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (Allergens)
RN  - 0 (Bee Venoms)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Adolescent
MH  - Allergens/immunology
MH  - Antibody Specificity
MH  - Bee Venoms/*immunology
MH  - Bites and Stings/*immunology
MH  - Child
MH  - Female
MH  - Histamine/*blood
MH  - *Histamine Release
MH  - Humans
MH  - Hypersensitivity/*diagnosis
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Male
MH  - Skin Tests/methods
MH  - Time Factors
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - 10.1034/j.1399-3038.1999.101012.x [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 1999 Feb;10(1):53-7. doi: 
      10.1034/j.1399-3038.1999.101012.x.