PMID- 10411089
OWN - NLM
STAT- MEDLINE
DCOM- 19990817
LR  - 20071114
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 162
IP  - 2
DP  - 1999 Aug
TI  - In vivo description of dendritic cells in human renal cell carcinoma.
PG  - 567-73
AB  - PURPOSE: Dendritic cells (DCs) are efficient and effective antigen-presenting 
      cells that play a major role in initiating the primary immune response. They are 
      the most potent stimulators of T-cell activation and would thus be expected to be 
      of great importance in the antitumoral immune response. Although DC phenotype and 
      function have been described under in vitro conditions, their in vivo 
      characteristics are less well detailed. Human renal cell carcinoma (RCC) is an 
      excellent model to explore tumor infiltrating dendritic cells (TiDCs) because of 
      rare clinical spontaneous regressions and the association of high numbers of 
      tumor infiltrating lymphocytes (TiLs), suggesting a strong immune response. 
      MATERIALS AND METHODS: We determined the in situ phenotype of mature CD83+ TiDCs 
      using monoclonal antibodies to known activation molecules (CD86 [B7.2], CD80 
      [B7.1], CD40, CD54, CD1a and HLA-DR). Seventeen primary RCCs, representing four 
      distinct histologies, were evaluated using double-staining immunohistochemical 
      techniques and light microscopy. RESULTS: CD83+ TiDCs were found in all tumors. 
      Expression of CD40 correlated with expression of CD1a on CD83+ TiDCs. Expression 
      of CD54 (ICAM-1) correlated with a lower expression of CD86 (B7.2) as well as a 
      decrease in CD3+ and CD8+ TiLs. CONCLUSIONS: These data suggest a de novo lipid 
      or sugar-based immunogenic antigen presentation by TiDCs. Also, the data support 
      an impaired antigen-presenting capability for CD54+ TiDCs based on the decreased 
      coexpression of CD86 (B7.2) and the decrease of associated CD8+ TiLs.
FAU - Schwaab, T
AU  - Schwaab T
AD  - Department of Hematology/Oncology, Medizinische Hochschule, Hannover, Germany.
FAU - Schned, A R
AU  - Schned AR
FAU - Heaney, J A
AU  - Heaney JA
FAU - Cole, B F
AU  - Cole BF
FAU - Atzpodien, J
AU  - Atzpodien J
FAU - Wittke, F
AU  - Wittke F
FAU - Ernstoff, M S
AU  - Ernstoff MS
LA  - eng
GR  - CCSG CA 23108/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 0 (Antigens, CD)
SB  - IM
CIN - J Urol. 1999 Aug;162(2):291-2. PMID: 10411024
MH  - Antigens, CD/analysis/*immunology
MH  - Carcinoma, Renal Cell/chemistry/*immunology/*pathology
MH  - Dendritic Cells/chemistry/*immunology/*pathology
MH  - Humans
MH  - Kidney Neoplasms/chemistry/*immunology/*pathology
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - S0022-5347(05)68628-4 [pii]
PST - ppublish
SO  - J Urol. 1999 Aug;162(2):567-73.