PMID- 10411545
OWN - NLM
STAT- MEDLINE
DCOM- 19990812
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 104
IP  - 2
DP  - 1999 Jul
TI  - Increased frequency of antigen-specific CD8(+) cytotoxic T lymphocytes 
      infiltrating an Epstein-Barr virus-associated gastric carcinoma.
PG  - 163-71
AB  - Gastric adenocarcinomas carrying Epstein-Barr virus (EBV) are known to be 
      accompanied by massive lymphocyte infiltration. To characterize the 
      tumor-infiltrating lymphocytes (TILs), we isolated and cultured such cells from a 
      surgically resected EBV-associated gastric carcinoma. They were found to be 
      positive for CD3, CD8, T-cell receptor beta chain, and cytotoxic molecules. The 
      isolated TILs consisted of human leukocyte antigen (HLA) class I-restricted 
      CD8(+) cytotoxic T lymphocytes (CTLs), which killed autologous EBV-transformed 
      cells (but not phytohemagglutinin blast cells) and recognized HLA-A24 as 
      restriction molecules. However, the TILs did not recognize known EBV antigenic 
      peptides presented by HLA-A24 molecules, nor HLA-A24(+) fibroblasts infected with 
      vaccinia recombinant virus expressing each of the EBV latent proteins. EBV(+) 
      gastric carcinomas do not express conventional target proteins of EBV-specific 
      CTLs, and the data suggest that some cellular proteins may be involved in the 
      strong T-cell response to EBV-associated gastric carcinoma. In addition, our data 
      suggest that class I-restricted, antigen-specific CD8(+) CTLs are specifically 
      expanded within EBV(+) gastric carcinoma tissue.
FAU - Kuzushima, K
AU  - Kuzushima K
AD  - Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Nagoya 
      464-0021, Japan. kkuzushi@aichi-cc.pref.aichi.jp
FAU - Nakamura, S
AU  - Nakamura S
FAU - Nakamura, T
AU  - Nakamura T
FAU - Yamamura, Y
AU  - Yamamura Y
FAU - Yokoyama, N
AU  - Yokoyama N
FAU - Fujita, M
AU  - Fujita M
FAU - Kiyono, T
AU  - Kiyono T
FAU - Tsurumi, T
AU  - Tsurumi T
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
SB  - IM
MH  - HLA-A Antigens/immunology
MH  - HLA-A24 Antigen
MH  - Herpesvirus 4, Human/*isolation & purification
MH  - Humans
MH  - Lymphocyte Activation
MH  - Lymphocytes, Tumor-Infiltrating/*immunology
MH  - Male
MH  - Middle Aged
MH  - Stomach Neoplasms/*immunology/pathology/virology
MH  - T-Lymphocytes, Cytotoxic/*immunology
PMC - PMC408473
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
PMCR- 1999/07/15
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
PHST- 1999/07/15 00:00 [pmc-release]
AID - 06062 [pii]
AID - 10.1172/JCI6062 [doi]
PST - ppublish
SO  - J Clin Invest. 1999 Jul;104(2):163-71. doi: 10.1172/JCI6062.