PMID- 10411546
OWN - NLM
STAT- MEDLINE
DCOM- 19990812
LR  - 20240508
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 104
IP  - 2
DP  - 1999 Jul
TI  - Rapid generation of broad T-cell immunity in humans after a single injection of 
      mature dendritic cells.
PG  - 173-80
AB  - Dendritic cells (DCs) are potent antigen-presenting cells that initiate 
      protective T-cell immunity in mice. To study the immunogenicity of DCs in humans, 
      we injected 9 healthy subjects subcutaneously with a control injection of 
      autologous monocyte-derived, mature DCs, followed 4-6 weeks later by DCs pulsed 
      with keyhole limpet hemocyanin (KLH), HLA-A*0201-positive restricted influenza 
      matrix peptide (MP), and tetanus toxoid (TT). Four more subjects received these 
      antigens without DCs. Injection of unpulsed DCs, or antigens alone, failed to 
      immunize. Priming of CD4(+) T cells to KLH was observed in all 9 subjects 
      injected with KLH-pulsed DCs, and boosting of TT-specific T-cell immunity was 
      seen in 5 of 6 subjects injected with TT-pulsed DCs. Injection of antigen-pulsed 
      DCs led to a severalfold increase in freshly isolated MP-specific, 
      IFN-gamma-secreting CD8(+) T cells in all 6 HLA-A*0201-positive subjects, as 
      early as 7 days after injection. When T cells were boosted in culture, there was 
      an increase in MHC tetramer-binding cells and cytotoxic T cells after DC 
      vaccination. These data provide the first controlled evidence of the 
      immunogenicity of DCs in humans, and demonstrate that a single injection of 
      mature DCs rapidly expands T-cell immunity.
FAU - Dhodapkar, M V
AU  - Dhodapkar MV
AD  - Laboratory of Immunology and Cellular Physiology, General Clinical Research 
      Center, and Aaron Diamond AIDS Research Center, Rockefeller University, New York, 
      New York 10021, USA. dhodapm@rockvax.rockefeller.edu
FAU - Steinman, R M
AU  - Steinman RM
FAU - Sapp, M
AU  - Sapp M
FAU - Desai, H
AU  - Desai H
FAU - Fossella, C
AU  - Fossella C
FAU - Krasovsky, J
AU  - Krasovsky J
FAU - Donahoe, S M
AU  - Donahoe SM
FAU - Dunbar, P R
AU  - Dunbar PR
FAU - Cerundolo, V
AU  - Cerundolo V
FAU - Nixon, D F
AU  - Nixon DF
FAU - Bhardwaj, N
AU  - Bhardwaj N
LA  - eng
GR  - R01 AI044628/AI/NIAID NIH HHS/United States
GR  - R01 AI040874/AI/NIAID NIH HHS/United States
GR  - M01 RR000102/RR/NCRR NIH HHS/United States
GR  - R01 AI044595/AI/NIAID NIH HHS/United States
GR  - AI-40874/AI/NIAID NIH HHS/United States
GR  - AI-44628/AI/NIAID NIH HHS/United States
GR  - AI-39516/AI/NIAID NIH HHS/United States
GR  - R37 AI044628/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (HLA-A Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - HLA-A Antigens/analysis
MH  - Humans
MH  - Immunization
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocytes/*immunology
PMC - PMC408478
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
PMCR- 1999/07/15
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
PHST- 1999/07/15 00:00 [pmc-release]
AID - 06909 [pii]
AID - 10.1172/JCI6909 [doi]
PST - ppublish
SO  - J Clin Invest. 1999 Jul;104(2):173-80. doi: 10.1172/JCI6909.