PMID- 10411686
OWN - NLM
STAT- MEDLINE
DCOM- 19990803
LR  - 20061115
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 56
IP  - 1
DP  - 1999 Jul
TI  - Monocyte chemoattractant protein-1 mediates collagen deposition in experimental 
      glomerulonephritis by transforming growth factor-beta.
PG  - 135-44
AB  - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) plays a significant role 
      in the recruitment of monocytes/macrophages in experimental glomerulonephritis 
      (GN). Because recent evidence points to possible profibrogenic effects of 
      leukocyte-derived factors in GN, this study was designed to evaluate the role of 
      the chemokine MCP-1 in the fibrogenesis of experimental GN. METHODS: Rats with an 
      anti-thy-1-induced GN were treated with a neutralizing antiserum against MCP-1. 
      Glomerular collagen type IV, as a marker of glomerular matrix deposition, was 
      assessed by Northern and Western blotting and immunohistology. Transforming 
      growth factor-beta (TGF-beta), an important mediator of this matrix expansion, 
      was studied by Northern and Western blotting. RESULTS: The induction of GN 
      resulted in a significant increase of glomerular collagen type IV deposition and 
      TGF-beta synthesis. The neutralization of MCP-1 significantly reduced the 
      enhanced collagen type IV protein synthesis and deposition without affecting 
      collagen mRNA expression. However, both the enhanced transcription and protein 
      synthesis of TGF-beta were inhibited by anti-MCP-1 antiserum in nephritic 
      animals. CONCLUSIONS: In this model of GN, MCP-1 has a fibrogenic effect through 
      the stimulation of TGF-beta. MCP-1 is thus not only important for the recruitment 
      of inflammatory cells, but also mediates glomerular matrix accumulation.
FAU - Schneider, A
AU  - Schneider A
AD  - Department of Medicine, University of Hamburg, Germany.
FAU - Panzer, U
AU  - Panzer U
FAU - Zahner, G
AU  - Zahner G
FAU - Wenzel, U
AU  - Wenzel U
FAU - Wolf, G
AU  - Wolf G
FAU - Thaiss, F
AU  - Thaiss F
FAU - Helmchen, U
AU  - Helmchen U
FAU - Stahl, R A
AU  - Stahl RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Chemokine CCL2)
RN  - 0 (Immune Sera)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cell Movement/physiology
MH  - Chemokine CCL2/immunology/*physiology
MH  - Collagen/*metabolism
MH  - Glomerulonephritis/*metabolism/pathology/physiopathology
MH  - Immune Sera/immunology
MH  - Macrophages/physiology
MH  - Male
MH  - Monocytes/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Transforming Growth Factor beta/*physiology
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - S0085-2538(15)46269-6 [pii]
AID - 10.1046/j.1523-1755.1999.00543.x [doi]
PST - ppublish
SO  - Kidney Int. 1999 Jul;56(1):135-44. doi: 10.1046/j.1523-1755.1999.00543.x.