PMID- 10412778
OWN - NLM
STAT- MEDLINE
DCOM- 19990915
LR  - 20220317
IS  - 0098-6577 (Print)
IS  - 0098-6577 (Linking)
VI  - 71
DP  - 1999 Jul
TI  - Rapid normalization of interleukin-8 production after low-density lipoprotein 
      apheresis in steroid-resistant nephrotic syndrome.
PG  - S210-2
AB  - BACKGROUND: Low-density lipoprotein apheresis (LDL-A) treatment combined with 
      steroids demonstrated significant improvement of nephrotic proteinuria in 
      steroid- or immunosuppressive-resistant patients from focal and segmental 
      glomerulosclerosis (FGS). The mechanisms of the effect of LDL-A in nephrotic 
      syndrome (NS) are unknown, but a reduction in inflammatory cytokines and 
      chemokines secreted from macrophages has been supposed. METHODS: Serum levels of 
      interleukin (IL)-8, tumor necrosis factor-alpha (TNF-alpha), and monocyte 
      chemoattractant protein-1 (MCP-1) were measured by enzyme-linked immunosorbent 
      assay in 27 patients with NS [13 with FGS and 14 with minimal change nephrotic 
      syndrome (MCNS)] before and after LDL-A and in 13 age-matched, healthy controls. 
      We also selected three FGS patients who were resistant to steroid therapy for at 
      least one month and who had undergone six LDL-A procedures. The effects of 
      steroids and LDL-A on the production of IL-8, TNF-alpha, and MCP-1 by peripheral 
      blood mononuclear cells (PBMCs) were also determined in some patients. RESULTS: 
      In NS, the serum levels of IL-8 and TNF-alpha, but not MCP-1, were significantly 
      higher than in healthy controls. After LDL-A, IL-8 and TNF-alpha tended to 
      decrease. IL-8 production by lipopolysaccharide (LPS)-stimulated PBMC, mainly 
      adherent cells, was significantly reduced in both the steroid-resistant FGS group 
      and nontreated NS group compared with controls, but TNF-alpha production was 
      reduced in the only FGS group. After LDL-A, only IL-8 production recovered to the 
      control group level. CONCLUSION: Significant amelioration of IL-8 production 
      independent of any effect of steroids on LPS-stimulated PBMCs may reflect a 
      beneficial effect of LDL-A in normalizing the function of circulating monocytes 
      in steroid-resistant FGS.
FAU - Sakurai, M
AU  - Sakurai M
AD  - Department of Cardiovascular Medicine, Kyoto University, Graduate School of 
      Medicine, Japan.
FAU - Muso, E
AU  - Muso E
FAU - Matushima, H
AU  - Matushima H
FAU - Ono, T
AU  - Ono T
FAU - Sasayama, S
AU  - Sasayama S
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PL  - United States
TA  - Kidney Int Suppl
JT  - Kidney international. Supplement
JID - 7508622
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Steroids)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - *Blood Component Removal
MH  - Chemokine CCL2/blood
MH  - Drug Resistance
MH  - Female
MH  - Glomerulonephritis, Membranous/blood/therapy
MH  - Glomerulosclerosis, Focal Segmental/blood/therapy
MH  - Humans
MH  - Interleukin-8/*blood
MH  - Leukocytes, Mononuclear/cytology/drug effects/metabolism
MH  - Lipoproteins, LDL/*blood
MH  - Male
MH  - Nephrotic Syndrome/blood/pathology/*therapy
MH  - Steroids/*therapeutic use
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 1999/07/21 00:00
MHDA- 1999/07/21 00:01
CRDT- 1999/07/21 00:00
PHST- 1999/07/21 00:00 [pubmed]
PHST- 1999/07/21 00:01 [medline]
PHST- 1999/07/21 00:00 [entrez]
AID - S0085-2538(15)46642-6 [pii]
PST - ppublish
SO  - Kidney Int Suppl. 1999 Jul;71:S210-2.