PMID- 10413116
OWN - NLM
STAT- MEDLINE
DCOM- 19990809
LR  - 20211203
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 454
IP  - 1-2
DP  - 1999 Jul 2
TI  - Examination of the signal transduction pathways leading to activation of 
      extracellular signal-regulated kinase by formyl-methionyl-leucyl-phenylalanine in 
      rat neutrophils.
PG  - 165-8
AB  - The signaling pathways leading to extracellular signal-regulated kinase (ERK) 
      activation in formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated rat 
      neutrophils were examined. fMLP-stimulated ERK activation based on immunoblot 
      analysis with antibodies against the phosphorylation form of ERK was attenuated 
      by the pretreatment of cells with pertussis toxin but not with a dual 
      cyclo-oxygenase/lipoxygenase inhibitor BW755C. Exposure of cells to the tyrosine 
      kinase inhibitor genistein, phosphatidylinositol 3-kinase (PI3K) inhibitors 
      wortmannin and LY294002, or protein kinase C (PKC) inhibitors Go6976, Go6983, and 
      GF109203X inhibited fMLP-stimulated ERK phosphorylation in a 
      concentration-dependent manner. In addition, both the phospholipase C (PLC) 
      inhibitor U73122 and the Ca2+ chelator BAPTA attenuated ERK activation. These 
      results indicate that G(i/o) protein, tyrosine kinase, P13K, PKC, and PLC/Ca2+, 
      but not arachidonate metabolites, act upstream of fMLP-stimulated ERK activation.
FAU - Chang, L C
AU  - Chang LC
AD  - Department of Education and Research, Taichung Veterans General Hospital, Taiwan, 
      ROC.
FAU - Wang, J P
AU  - Wang JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Enzyme Inhibitors)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - GTP-Binding Proteins/physiology
MH  - Mitogen-Activated Protein Kinase Kinases
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/*enzymology
MH  - Protein Kinase C/antagonists & inhibitors
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Rats
MH  - *Signal Transduction
MH  - Type C Phospholipases/metabolism
EDAT- 1999/07/21 00:00
MHDA- 1999/07/21 00:01
CRDT- 1999/07/21 00:00
PHST- 1999/07/21 00:00 [pubmed]
PHST- 1999/07/21 00:01 [medline]
PHST- 1999/07/21 00:00 [entrez]
AID - S0014-5793(99)00717-6 [pii]
AID - 10.1016/s0014-5793(99)00717-6 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jul 2;454(1-2):165-8. doi: 10.1016/s0014-5793(99)00717-6.