PMID- 10413455
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20161124
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 40
IP  - 2
DP  - 1999 Aug
TI  - BDNF and NT4/5 promote survival and neurite outgrowth of pontocerebellar mossy 
      fiber neurons.
PG  - 254-69
AB  - The neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor 
      (BDNF), neurotrophin-3 (NT3), and NT4/5 are all found in the developing 
      cerebellum. Granule cells, the major target neurons of mossy fibers, express BDNF 
      during mossy fiber synaptogenesis. To determine whether neurotrophins contribute 
      to the development of cerebellar afferent axons, we characterized the effects of 
      neurotrophins on the growth of mossy fiber neurons from mice and rats in vitro. 
      For a mossy fiber source, we used the basilar pontine nuclei (BPN), the major 
      source of cerebellar mossy fibers in mammals. BDNF and NT4/5 increased BPN neuron 
      survival, neurite outgrowth, growth cone size, and elongation rate, while neither 
      NT3 nor NGF increased survival or outgrowth. In addition, BDNF and NT4/5 reduced 
      the size of neurite bundles. Consistent with these effects, in situ hybridization 
      on cultured basilar pontine neurons revealed the presence of mRNA encoding the 
      TrkB receptor which binds both BDNF and NT4/5 with high affinity. We detected 
      little or no message encoding the TrkC receptor which preferentially binds NT3. 
      BDNF and NT4/5 also increased TrkB mRNA levels in BPN neurons. In addition to 
      previously established functions as an autocrine/paracrine trophic factor for 
      granule cells, the present results indicate that cerebellar BDNF may also act as 
      a target-derived trophic factor for basilar pontine mossy fibers.
CI  - Copyright 1999 John Wiley & Sons, Inc.
FAU - Rabacchi, S A
AU  - Rabacchi SA
AD  - Department of Neurobiology and Anatomy, MCP Hahnemann School of Medicine, MCP 
      Hahnemann University, 3200 Henry Avenue, Philadelphia, Pennsylvania 19129, USA.
FAU - Kruk, B
AU  - Kruk B
FAU - Hamilton, J
AU  - Hamilton J
FAU - Carney, C
AU  - Carney C
FAU - Hoffman, J R
AU  - Hoffman JR
FAU - Meyer, S L
AU  - Meyer SL
FAU - Springer, J E
AU  - Springer JE
FAU - Baird, D H
AU  - Baird DH
LA  - eng
GR  - HD07467/HD/NICHD NIH HHS/United States
GR  - NS33214/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 145172-44-7 (neurotrophin 5)
RN  - EC 2.7.10.1 (Receptor, trkC)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Afferent Pathways
MH  - Animals
MH  - Axons/drug effects
MH  - Brain-Derived Neurotrophic Factor/*pharmacology/physiology
MH  - Cell Survival/drug effects
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Fibers/*drug effects/ultrastructure
MH  - Nerve Growth Factors/*pharmacology
MH  - Neurites/*drug effects
MH  - Neurons/metabolism
MH  - Pons/*cytology/growth & development
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptor, Ciliary Neurotrophic Factor/analysis
MH  - Receptor, trkC/analysis
MH  - Recombinant Fusion Proteins/pharmacology
EDAT- 1999/07/21 00:00
MHDA- 1999/07/21 00:01
CRDT- 1999/07/21 00:00
PHST- 1999/07/21 00:00 [pubmed]
PHST- 1999/07/21 00:01 [medline]
PHST- 1999/07/21 00:00 [entrez]
AID - 10.1002/(SICI)1097-4695(199908)40:2<254::AID-NEU11>3.0.CO;2-4 [pii]
PST - ppublish
SO  - J Neurobiol. 1999 Aug;40(2):254-69.