PMID- 10414366
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 67
IP  - 2
DP  - 1999 Jun 15
TI  - Development and evaluation of an immunomagnetic separation-ELISA for the 
      detection of Staphylococcus aureus thermostable nuclease in composite milk.
PG  - 113-25
AB  - An ELISA method based on monodisperse magnetic beads was developed for the 
      detection of Staphylococcus aureus thermostable nuclease (TNase) in composite 
      milk, wherein S. aureus TNase is captured by magnetic beads coated with 
      monoclonal antibodies directed against TNase and subsequently detected by an 
      enzyme-labelled MAb against the same antigen. Sensitivity of the test was 
      approximately 1 ng TNase, which corresponds to the amount of TNase produced and 
      secreted by approximately 10(5) S. aureus per ml. The Immuno Magnetic Separation 
      (IMS)-ELISA detected TNase in samples from which no S. aureus could be 
      demonstrated on culture. The total test time is 3 h and can be performed either 
      on preserved or fresh milk. The method may be automated.
FAU - Yazdankhah, S P
AU  - Yazdankhah SP
AD  - Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of 
      Veterinary Medicine, Oslo. siamak.p.yazdankhah@veths.no
FAU - Solverod, L
AU  - Solverod L
FAU - Simonsen, S
AU  - Simonsen S
FAU - Olsen, E
AU  - Olsen E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Coloring Agents)
RN  - 15XUH0X66N (Tolonium Chloride)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Cattle
MH  - Coloring Agents/chemistry
MH  - Enzyme-Linked Immunosorbent Assay/methods/veterinary
MH  - Female
MH  - Immunomagnetic Separation/methods/veterinary
MH  - Mastitis, Bovine/*diagnosis/immunology
MH  - Micrococcal Nuclease/analysis/chemistry
MH  - Microspheres
MH  - Milk/microbiology
MH  - Sensitivity and Specificity
MH  - Staphylococcal Infections/diagnosis/immunology/*veterinary
MH  - Staphylococcus aureus/immunology/*isolation & purification
MH  - Tolonium Chloride/chemistry
EDAT- 1999/07/22 00:00
MHDA- 1999/07/22 00:01
CRDT- 1999/07/22 00:00
PHST- 1999/07/22 00:00 [pubmed]
PHST- 1999/07/22 00:01 [medline]
PHST- 1999/07/22 00:00 [entrez]
AID - S0378113599000358 [pii]
AID - 10.1016/s0378-1135(99)00035-8 [doi]
PST - ppublish
SO  - Vet Microbiol. 1999 Jun 15;67(2):113-25. doi: 10.1016/s0378-1135(99)00035-8.