PMID- 10416379
OWN - NLM
STAT- MEDLINE
DCOM- 19990812
LR  - 20200324
IS  - 0304-8608 (Print)
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 144
IP  - 5
DP  - 1999
TI  - Determination of 5'-leader sequences from radically disparate strains of porcine 
      reproductive and respiratory syndrome virus reveals the presence of highly 
      conserved sequence motifs.
PG  - 981-7
AB  - We determined the untranslated 5'-leader sequence for three different isolates of 
      porcine reproductive and respiratory syndrome virus (PRRSV): pathogenic European- 
      and American-types, as well as an American-type vaccine strain. 5'-leader from 
      European- and American-type PRRSV differed in length (220 and 190 nt, 
      respectively), and exhibited only approximately 50% nucleotide homology. 
      Nevertheless, highly conserved areas were identified in the leader of all 3 PRRSV 
      isolates, which constitute candidate motifs for binding of protein(s) involved in 
      viral replication. These comparative data provide a priori knowledge for 
      mutational identification of virulence determinants in the 5' nontranslated part 
      of the PRRSV genome.
FAU - Oleksiewicz, M B
AU  - Oleksiewicz MB
AD  - Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark.
FAU - Botner, A
AU  - Botner A
FAU - Nielsen, J
AU  - Nielsen J
FAU - Storgaard, T
AU  - Storgaard T
LA  - eng
SI  - GENBANK/AF094475
SI  - GENBANK/AF094476
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions/*genetics
MH  - Animals
MH  - Base Sequence
MH  - Conserved Sequence
MH  - Europe
MH  - Molecular Sequence Data
MH  - North America
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification/physiology
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Swine
MH  - Virus Replication
PMC - PMC7086836
EDAT- 1999/07/23 00:00
MHDA- 1999/07/23 00:01
PMCR- 2020/03/23
CRDT- 1999/07/23 00:00
PHST- 1999/07/23 00:00 [pubmed]
PHST- 1999/07/23 00:01 [medline]
PHST- 1999/07/23 00:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 91440981 [pii]
AID - 10.1007/s007050050560 [doi]
PST - ppublish
SO  - Arch Virol. 1999;144(5):981-7. doi: 10.1007/s007050050560.