PMID- 10419598
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20220409
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 188
IP  - 3
DP  - 1999 Jul
TI  - Cyclin D1 expression in astrocytomas is associated with cell proliferation 
      activity and patient prognosis.
PG  - 289-93
AB  - An important positive regulator of the cell cycle, cyclin D1, is often amplified 
      and overexpressed in malignancies. Cyclin D1 aberrations were analysed in grade 
      II-IV astrocytomas by fluorescence in situ hybridization (FISH), mRNA in situ 
      hybridization and immunohistochemistry. Proliferation activity was determined by 
      Ki-67(MIB-1) immunolabelling and mitotic counting. High cyclin D1 expression was 
      observed in grade IV astrocytomas (grades II-III versus grade IV; mRNA 
      expression: p<0.001; immunoexpression: p=0.013), and correlated with poor patient 
      survival (p<0.001, n=46). Upregulated cyclin D1 expression was also closely 
      associated with poor patient prognosis in grade II-III astrocytomas (p<0.001, 
      n=30). Cyclin D1 gene was not found to be amplified (n=7). Cell proliferation 
      activity was significantly increased in tumours exhibiting high cyclin D1 mRNA 
      levels (Ki-67(MIB-1): p<0.001; mitotic count: p<0.001) and high cyclin D1 protein 
      expression (Ki-67(MIB-1): p=0.002; mitotic count: p=0.012). These results 
      indicate that increased production of cyclin D1 is closely associated with high 
      cell proliferation activity and aggressive behaviour in diffusely infiltrating 
      astrocytomas.
CI  - Copyright 1999 John Wiley & Sons, Ltd.
FAU - Sallinen, S L
AU  - Sallinen SL
AD  - Department of Pathology, Tampere University Hospital, Finland. losasa@uta.fi
FAU - Sallinen, P K
AU  - Sallinen PK
FAU - Kononen, J T
AU  - Kononen JT
FAU - Syrjakoski, K M
AU  - Syrjakoski KM
FAU - Nupponen, N N
AU  - Nupponen NN
FAU - Rantala, I S
AU  - Rantala IS
FAU - Helen, P T
AU  - Helen PT
FAU - Helin, H J
AU  - Helin HJ
FAU - Haapasalo, H K
AU  - Haapasalo HK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Astrocytoma/*metabolism/pathology
MH  - Biomarkers, Tumor/*metabolism
MH  - Cell Division
MH  - Cyclin D1/genetics/*metabolism
MH  - Follow-Up Studies
MH  - Gene Expression
MH  - Humans
MH  - Neoplasm Proteins/*metabolism
MH  - Prognosis
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics
MH  - Survival Rate
EDAT- 1999/07/27 10:00
MHDA- 2000/03/04 09:00
CRDT- 1999/07/27 10:00
PHST- 1999/07/27 10:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 1999/07/27 10:00 [entrez]
AID - 10.1002/(SICI)1096-9896(199907)188:3<289::AID-PATH351>3.0.CO;2-X [pii]
AID - 10.1002/(SICI)1096-9896(199907)188:3<289::AID-PATH351>3.0.CO;2-X [doi]
PST - ppublish
SO  - J Pathol. 1999 Jul;188(3):289-93. doi: 
      10.1002/(SICI)1096-9896(199907)188:3<289::AID-PATH351>3.0.CO;2-X.