PMID- 10419874
OWN - NLM
STAT- MEDLINE
DCOM- 19990817
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 94
IP  - 3
DP  - 1999 Aug 1
TI  - Serial analysis of gene expression in human monocyte-derived dendritic cells.
PG  - 845-52
AB  - Dendritic cells (DCs) are professional antigen-presenting cells in the immune 
      system and can be generated in vitro from hematopoietic progenitor cells in the 
      bone marrow, CD34(+) cord blood cells, precursor cells in the peripheral blood, 
      and blood monocytes by culturing with granulocyte-macrophage colony-stimulating 
      factor (GM-CSF), interleukin-4, and tumor necrosis factor-alpha. We have 
      performed serial analysis of gene expression (SAGE) in DCs derived from human 
      blood monocytes. A total of 58,540 tag sequences from a DC complementary DNA 
      (cDNA) library represented more than 17,000 different genes, and these data were 
      compared with SAGE analysis of tags from monocytes (Mo) and GM-CSF-induced 
      macrophages (M open diamond). Many of the genes that were differentially 
      expressed in DCs were identified as genes encoding proteins related to cell 
      structure and cell motility. Interestingly, the highly expressed genes in DCs 
      encode chemokines such as TARC, MDC, and MCP-4, which preferentially chemoattract 
      Th2-type lymphocytes. Although DCs have been considered to be very heterogeneous, 
      the identification of specific genes expressed in human Mo-derived DCs should 
      provide candidate genes to define subsets of, the function of, and the maturation 
      stage of DCs and possibly also to diagnose diseases in which DCs play a 
      significant role, such as autoimmune diseases and neoplasms. This study 
      represents the first extensive gene expression analysis in any type of DCs.
FAU - Hashimoto, S
AU  - Hashimoto S
AD  - Department of Molecular Preventive Medicine and CREST, School of Medicine, The 
      University of Tokyo, Tokyo, Japan.
FAU - Suzuki, T
AU  - Suzuki T
FAU - Dong, H Y
AU  - Dong HY
FAU - Nagai, S
AU  - Nagai S
FAU - Yamazaki, N
AU  - Yamazaki N
FAU - Matsushima, K
AU  - Matsushima K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Chemokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antigen Presentation/genetics
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Chemokines/*genetics/immunology
MH  - Dendritic Cells/*physiology
MH  - *Gene Expression
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interleukin-4/pharmacology
MH  - Monocytes/cytology/immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
AID - S0006-4971(20)67382-7 [pii]
PST - ppublish
SO  - Blood. 1999 Aug 1;94(3):845-52.