PMID- 10420388
OWN - NLM
STAT- MEDLINE
DCOM- 19991013
LR  - 20191103
IS  - 0379-0355 (Print)
IS  - 0379-0355 (Linking)
VI  - 21
IP  - 5
DP  - 1999 Jun
TI  - Effects of Cerebrolysin on in vitro primary microglial and astrocyte rat cell 
      cultures.
PG  - 331-8
AB  - In recent years the potential use of neurotrophic factors in the prevention 
      and/or treatment of neurodegenerative diseases has received much attention. To 
      determine whether Cerebrolysin, a porcine brain-derived peptide preparation, was 
      able to modulate in vitro lipopolysaccharide (LPS)-induced microglial activation 
      and to test the direct effect of Cerebrolysin on astrocyte morphology, survival 
      and proliferation, rat glial and astrocyte cell culture experiments were carried 
      out. The morphology of microglia, ameboid/activated and flat/resting, was 
      examined under contrast microscopy and cell counts obtained. In addition, the 
      release of interleukin (IL)-1 beta and brain-derived neurotrophic factor (BDNF) 
      was measured from cell culture supernatant using an enzyme-linked-immunoassay 
      (ELISA). The results obtained in this study clearly suggest a protective effect 
      of Cerebrolysin as revealed by downregulation of microglial activation after LPS 
      treatment as well as by the control of IL-1 beta expression. No significant 
      differences were observed on astrocyte morphology, survival or the production 
      and/or release of BDNF. In conclusion, these in vitro studies indicate that 
      Cerebrolysin might exert a neuroimmunotrophic function which can in turn reduce 
      the extent of inflammation and accelerate neuronal death under pathological 
      conditions such as human neurodegenerative disorders.
FAU - Lombardi, V R
AU  - Lombardi VR
AD  - Biotechnology Division, EuroEspes, Basic and Clinical Neurosciences Research 
      Center, La Coruna, Spain. euroespes@lcg.servicom.es
FAU - Windisch, M
AU  - Windisch M
FAU - Garcia, M
AU  - Garcia M
FAU - Cacabelos, R
AU  - Cacabelos R
LA  - eng
PT  - Journal Article
PL  - Spain
TA  - Methods Find Exp Clin Pharmacol
JT  - Methods and findings in experimental and clinical pharmacology
JID - 7909595
RN  - 0 (Amino Acids)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Interleukin-1)
RN  - 0 (Nootropic Agents)
RN  - 37KZM6S21G (cerebrolysin)
SB  - IM
MH  - Amino Acids/*pharmacology
MH  - Animals
MH  - Astrocytes/*drug effects
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Count
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Immunohistochemistry
MH  - Interleukin-1/metabolism
MH  - Microglia/*drug effects
MH  - Nootropic Agents/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Swine
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
AID - 541910 [pii]
AID - 10.1358/mf.1999.21.5.541910 [doi]
PST - ppublish
SO  - Methods Find Exp Clin Pharmacol. 1999 Jun;21(5):331-8. doi: 
      10.1358/mf.1999.21.5.541910.