PMID- 10421428
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 65
IP  - 4
DP  - 1999
TI  - Acteoside inhibits apoptosis in D-galactosamine and lipopolysaccharide-induced 
      liver injury.
PG  - 421-30
AB  - We assessed the effect of acteoside, a naturally occurring antioxidative 
      phenylethanoid, on hepatic apoptosis and the subsequent liver failure induced by 
      D-Galactosamine (D-GalN) and lipopolysaccharide (LPS). A co-administration of 
      D-GalN (700 mg/kg) and LPS (35 microg/kg) to mice evoked typical hepatic 
      apoptosis characterized by DNA fragmentation and apoptotic body formation, 
      resulting in fulminant hepatitis and lethality of mice. Pre-administration of 
      acteoside at 10 or 50 mg/kg subcutaneously at 12 and 1 h prior to D-GalN/LPS 
      intoxication significantly inhibited hepatic apoptosis, hepatitis and lethality. 
      Tumor necrosis factor-alpha (TNF-alpha) secreted from LPS-stimulated macrophages 
      is an important mediator of apoptosis in this model. Acteoside showed no apparent 
      effect on the marked elevation of serum TNF-alpha, but it partially prevented in 
      vitro TNF-alpha (100 ng/ml)-induced cell death in D-GalN (0.5 mM)-sensitized 
      hepatocytes at the concentrations of 50, 100 and 200 microM. These results 
      indicated that D-GalN/LPS-induced hepatic apoptosis can be blocked by an 
      exogenous antioxidant, suggesting the involvement of reactive oxygen 
      intermediates (ROIs) in TNF-alpha-dependent hepatic apoptosis.
FAU - Xiong, Q
AU  - Xiong Q
AD  - Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama 
      Medical & Pharmaceutical University, Japan.
FAU - Hase, K
AU  - Hase K
FAU - Tezuka, Y
AU  - Tezuka Y
FAU - Namba, T
AU  - Namba T
FAU - Kadota, S
AU  - Kadota S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Antioxidants)
RN  - 0 (Glucosides)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3TGX09BD5B (acteoside)
RN  - 7535-00-4 (Galactosamine)
RN  - 9007-49-2 (DNA)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Aspartate Aminotransferases/blood
MH  - Cell Survival
MH  - Chemical and Drug Induced Liver Injury/blood/pathology/*prevention & control
MH  - DNA/drug effects
MH  - Drug Synergism
MH  - Galactosamine/toxicity
MH  - Glucosides/*pharmacology
MH  - Lipopolysaccharides/toxicity
MH  - Liver/*drug effects/pathology
MH  - Liver Failure/blood/chemically induced/pathology/*prevention & control
MH  - Male
MH  - Mice
MH  - *Phenols
MH  - Plant Extracts/pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism/pharmacology
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
AID - S0024320599002635 [pii]
AID - 10.1016/s0024-3205(99)00263-5 [doi]
PST - ppublish
SO  - Life Sci. 1999;65(4):421-30. doi: 10.1016/s0024-3205(99)00263-5.