PMID- 10423398
OWN - NLM
STAT- MEDLINE
DCOM- 19990907
LR  - 20071114
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 21
IP  - 2
DP  - 1999 Aug
TI  - Forkhead transcription factor HFH-4 expression is temporally related to 
      ciliogenesis.
PG  - 168-76
AB  - Members of the forkhead/winged-helix family of transcription factors are 
      expressed in tissue-specific patterns and play critical roles in development and 
      cell differentiation. The expression of forkhead family member hepatocyte nuclear 
      factor-3/forkhead homologue 4 (HFH-4) has been localized by RNA-blot analysis and 
      in situ hybridization to the proximal airway of the lung (trachea, bronchi, and 
      bronchioles) with onset at mouse embryonic day (E) 14.5 and is present in the 
      choroid plexus, ependymal cells, oviduct, and testis. We hypothesized that the 
      restricted expression of HFH-4 messenger RNA suggests a function common to these 
      tissues and therefore a cell-specific role for HFH-4. Accordingly, an anti-HFH-4 
      antibody was generated and used for cell-specific localization of protein 
      expression to begin to identify the functions of HFH-4. We found HFH-4 expression 
      in proximal airway ciliated epithelial cells, but not Clara cells or alveolar 
      epithelial cells. HFH-4 was also expressed in ciliated epithelial cells of the 
      nose and paranasal sinuses, choroid plexus, ependyma, and oviduct. In developing 
      mouse lung, HFH-4 expression was initially detected in airway epithelial cells at 
      E15.5, before the appearance of cilia, and at later stages was localized to 
      epithelial cells with cilia. In the testis, HFH-4 expression in spermatids was 
      coincident with stage-specific generation of flagella. The temporal relationship 
      of HFH-4 expression to the development of cilia and flagella, and the restricted 
      expression in ciliated epithelial cells, suggest that this transcription factor 
      has a role in regulation and maintenance of the ciliated cell phenotype in 
      epithelial cells.
FAU - Blatt, E N
AU  - Blatt EN
AD  - Department of Internal Medicine, Washington University School of Medicine, St. 
      Louis, Missouri, USA.
FAU - Yan, X H
AU  - Yan XH
FAU - Wuerffel, M K
AU  - Wuerffel MK
FAU - Hamilos, D L
AU  - Hamilos DL
FAU - Brody, S L
AU  - Brody SL
LA  - eng
GR  - R29-HL56244/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hepatocyte Nuclear Factor 4)
RN  - 0 (MLX protein, human)
RN  - 0 (Phosphoproteins)
RN  - 0 (Tcfl4 protein, mouse)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
MH  - Brain/anatomy & histology
MH  - Cell Division
MH  - Cilia/*metabolism/physiology
MH  - DNA, Complementary/analysis
MH  - *DNA-Binding Proteins
MH  - Epithelial Cells/cytology
MH  - Gene Expression
MH  - Hepatocyte Nuclear Factor 4
MH  - Humans
MH  - Immunohistochemistry
MH  - Lung/anatomy & histology/embryology
MH  - Male
MH  - Mice
MH  - Nose/anatomy & histology
MH  - Oviducts/anatomy & histology
MH  - Paranasal Sinuses/anatomy & histology
MH  - Phosphoproteins/immunology/*metabolism
MH  - Rats
MH  - Spermatids/metabolism
MH  - Testis/anatomy & histology
MH  - Time Factors
MH  - Transcription Factors/immunology/*metabolism
EDAT- 1999/07/29 00:00
MHDA- 1999/07/29 00:01
CRDT- 1999/07/29 00:00
PHST- 1999/07/29 00:00 [pubmed]
PHST- 1999/07/29 00:01 [medline]
PHST- 1999/07/29 00:00 [entrez]
AID - 10.1165/ajrcmb.21.2.3691 [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 1999 Aug;21(2):168-76. doi: 10.1165/ajrcmb.21.2.3691.