PMID- 10423614
OWN - NLM
STAT- MEDLINE
DCOM- 20000124
LR  - 20191103
IS  - 1075-2765 (Print)
IS  - 1075-2765 (Linking)
VI  - 4
IP  - 7-8
DP  - 1997 Jul-Aug
TI  - Control of mast-cell 5-lipoxygenase.
PG  - 225-8
AB  - Leukotrienes (LTs) from mast cells make important contributions to early events 
      in inflammation. Therefore, the control of their 5-lipoxygenase was studied. We 
      observed that culture conditions can significantly alter LT synthesis and 
      intracellular 5-lipoxygenase levels. Challenge of mast cells with calcium 
      ionophore A23187 and immunoglobin E (IgE)/antigen had different effects. The 
      calcium ionophore caused continuous LT formation that was accompanied by the 
      translocation of the enzyme to membranes as well as substantial loss of activity. 
      In contrast, with receptor-mediated stimulation, IgE/antigen, enzyme inactivation 
      was insignificant, and membrane binding and LT synthesis were transient. Addition 
      of a calcium chelator stopped ionophore-induced LT production and inactivation of 
      the 5-lipoxygenase and reversed its membrane association. Therefore, 
      receptor-mediated activation of 5-lipoxygenase differs from that by calcium 
      ionophore. The data also indicate that calcium regulates the membrane binding and 
      dissociation of the 5-lipoxygenase. However, with excessively high calcium 
      concentrations activity is lost.
FAU - Malaviya, R
AU  - Malaviya R
AD  - Department of Molecular Biology and Pharmacology, Washngton University School of 
      Medicine, St. Louis, MO, USA.
FAU - Malaviya, R
AU  - Malaviya R
FAU - Jakschik, B A
AU  - Jakschik BA
LA  - eng
GR  - HL21874/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Ther
JT  - American journal of therapeutics
JID - 9441347
RN  - 0 (Leukotrienes)
RN  - 37H9VM9WZL (Calcimycin)
RN  - EC 1.13.11.34 (Arachidonate 5-Lipoxygenase)
SB  - IM
MH  - Animals
MH  - Arachidonate 5-Lipoxygenase/*metabolism
MH  - Calcimycin/pharmacology
MH  - Cells, Cultured
MH  - Leukotrienes/biosynthesis
MH  - Mast Cells/*enzymology
MH  - Mice
EDAT- 1997/07/01 00:00
MHDA- 1999/07/29 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1999/07/29 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1097/00045391-199707000-00002 [doi]
PST - ppublish
SO  - Am J Ther. 1997 Jul-Aug;4(7-8):225-8. doi: 10.1097/00045391-199707000-00002.