PMID- 10423733
OWN - NLM
STAT- MEDLINE
DCOM- 19990913
LR  - 20110225
IS  - 1121-7138 (Print)
IS  - 1121-7138 (Linking)
VI  - 22
IP  - 3
DP  - 1999 Jul
TI  - Inhibition of human immunodeficiency virus 1 (HIV-1) by variant B of human 
      herpesvirus 6 (HHV-6).
PG  - 161-71
AB  - Four HHV-6 strains were initially isolated during attempts to observe HIV-1 
      replication in cultured primary lymphocytes from 48 patients with AIDS. HHV-6 DNA 
      from each strain was extracted from primary cell cultures and amplified using 
      specific primers in a nested polymerase chain reaction (PCR) assay. All HHV-6 
      strains were classified as B variants by submitting the PCR products to the 
      digestion of two restriction enzymes (Hind III and Bgl II). Since in primary 
      cultures, the appearance of HHV-6 cytopathic effect was followed by a progressive 
      reduction of HIV-1 replication, we tried to reproduce the observed inhibition in 
      vitro. Two HHV-6 strains, used throughout the experiments, showed their ability 
      to suppress HIV-1 replication when the viruses co-infected CD4+T lymphocyte 
      cultures. While the intrinsic mechanism of this finding still remains unclear, 
      the inhibition of HIV-1 replication was observed only when a high multiplicity of 
      infection (m.o.i.) of HHV-6 and a low m.o.i. of HIV-1 were used in dually 
      infected cell cultures. By using a semiquantitative determination of HIV-1 cDNA 
      by PCR, it appears that the inhibition begins in infected cell cultures and, once 
      established, does not allow any further HIV-1 replication.
FAU - Bonura, F
AU  - Bonura F
AD  - Dipartimento di Igiene e Microbiologia G. D'Alessandro, Facolta di Medicina e 
      Chirurgia, Universita degli Studi, Palermo, Italia.
FAU - Perna, A M
AU  - Perna AM
FAU - Vitale, F
AU  - Vitale F
FAU - Villafrate, M R
AU  - Villafrate MR
FAU - Viviano, E
AU  - Viviano E
FAU - Guttadauro, R
AU  - Guttadauro R
FAU - Mazzola, G
AU  - Mazzola G
FAU - Romano, N
AU  - Romano N
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - New Microbiol
JT  - The new microbiologica
JID - 9516291
RN  - 0 (DNA, Viral)
RN  - 0 (HIV Core Protein p24)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*virology
MH  - CD4-Positive T-Lymphocytes/virology
MH  - Cell Death
MH  - Cytopathogenic Effect, Viral
MH  - DNA, Viral/isolation & purification
MH  - HIV Core Protein p24/analysis
MH  - HIV-1/*growth & development
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 6, Human/classification/genetics/*growth & development
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - *Viral Interference
MH  - Virus Replication
EDAT- 1999/07/29 00:00
MHDA- 1999/07/29 00:01
CRDT- 1999/07/29 00:00
PHST- 1999/07/29 00:00 [pubmed]
PHST- 1999/07/29 00:01 [medline]
PHST- 1999/07/29 00:00 [entrez]
PST - ppublish
SO  - New Microbiol. 1999 Jul;22(3):161-71.