PMID- 10425190
OWN - NLM
STAT- MEDLINE
DCOM- 19990908
LR  - 20051121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 2
DP  - 1999 Aug 2
TI  - Heat shock cognate protein 70 is a cell fusion-enhancing factor but not an entry 
      factor for human T-cell lymphotropic virus type I.
PG  - 357-63
AB  - Heat shock cognate protein 70 (HSC70) has been shown to bind to the peptide 
      corresponding to amino acids 197 to 216 of human T-cell lymphotropic virus type I 
      (HTLV-I) envelope protein, gp46, and an anti-HSC70 monoclonal antibody (mAb) 
      inhibits HTLV-I-induced syncytium formation. These findings suggest that HSC70 is 
      necessary for the entry of HTLV-I into its target cells. Here we showed that 
      HSC70 directly binds to gp46 by co-immunoprecipitation of HSC70 and gp46 from 
      HTLV-I-producing human T-cell lysate. However, transduction of human HSC70 cDNA 
      into BaF3 cells, which were found to be highly resistant to HTLV-I infection, did 
      not support the HTLV-I entry, and HSC70 expressed in NIH3T3 cells, which were 
      found to be almost resistant to syncytium formation upon cocultivation with 
      HTLV-I-producing cells but sensitive to infection with cell-free HTLV-I, enhanced 
      cell fusion induced by HTLV-I-producing cells, but did not enhance the entry of 
      cell-free HTLV-I into these cells. The mAb against HSC70 inhibited syncytium 
      formation in NIH3T3 cells expressing HSC70, but showed little effect on infection 
      of these cells with cell-free HTLV-I. These findings indicate that HSC70 markedly 
      enhances syncytium formation induced by HTLV-I but does not facilitate HTLV-I 
      entry into target cells.
CI  - Copyright 1999 Academic Press.
FAU - Fang, D
AU  - Fang D
AD  - Department of Virology and Preventive Medicine, Gunma University School of 
      Medicine, Showa-machi, Maebashi, Gunma, 371-8511, Japan.
FAU - Haraguchi, Y
AU  - Haraguchi Y
FAU - Jinno, A
AU  - Jinno A
FAU - Soda, Y
AU  - Soda Y
FAU - Shimizu, N
AU  - Shimizu N
FAU - Hoshino, H
AU  - Hoshino H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Gene Products, env)
RN  - 0 (HSC70 Heat-Shock Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (HSPA8 protein, human)
RN  - 0 (Hspa8 protein, mouse)
RN  - 0 (Hspa8 protein, rat)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (gp46 protein, Human T-cell leukemia virus type I)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Base Sequence
MH  - Carrier Proteins/genetics/immunology/*physiology
MH  - Cats
MH  - Cell Fusion/*physiology
MH  - Cell Line
MH  - DNA Primers/genetics
MH  - Gene Products, env/genetics/physiology
MH  - Giant Cells/pathology/physiology
MH  - HSC70 Heat-Shock Proteins
MH  - *HSP70 Heat-Shock Proteins
MH  - Human T-lymphotropic virus 1/genetics/*pathogenicity
MH  - Humans
MH  - Mice
MH  - Rats
MH  - Retroviridae Proteins, Oncogenic/genetics/physiology
MH  - Transduction, Genetic
EDAT- 1999/07/30 00:00
MHDA- 1999/07/30 00:01
CRDT- 1999/07/30 00:00
PHST- 1999/07/30 00:00 [pubmed]
PHST- 1999/07/30 00:01 [medline]
PHST- 1999/07/30 00:00 [entrez]
AID - S0006-291X(99)91028-8 [pii]
AID - 10.1006/bbrc.1999.1028 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 2;261(2):357-63. doi: 
      10.1006/bbrc.1999.1028.