PMID- 10425445
OWN - NLM
STAT- MEDLINE
DCOM- 19990922
LR  - 20191024
IS  - 0952-5041 (Print)
IS  - 0952-5041 (Linking)
VI  - 23
IP  - 1
DP  - 1999 Aug
TI  - Structure and expression of the mouse growth hormone receptor/growth hormone 
      binding protein gene.
PG  - 33-44
AB  - The mouse growth hormone receptor/growth hormone-binding protein (GHR/BP) gene 
      produces several distinct mRNA forms through alternative splicing, including 
      mRNAs encoding the membrane-bound growth hormone receptor (GHR) and the soluble 
      growth hormone-binding protein (GHBP). Transcripts are also heterogeneous in 
      their 5' regions due to alternative selection of two major 5' untranslated region 
      (5'UTR) sequences, designated L1 and L2. Here we report the cloning of all mouse 
      GHR/BP coding exons as well as the exon encoding 5'UTR L2, the most widely 
      expressed 5'UTR. The mouse GHR/GHBP gene contains 11 coding exons, 9 of which are 
      homologous in size and sequence to human GHR exons 2-10. The two mouse exons that 
      do not have homologs in the human gene are designated exons 4B and 8A. Exon 4B, 
      located between exons 4 and 5, encodes an 8-amino acid segment of the ligand 
      binding domain that is unique to mouse GHR and GHBP. Analysis by reverse 
      transcriptase-polymerase chain reaction indicated that exon 4B is constitutively 
      present in mouse GHR and GHBP mRNA. Exon 8A encodes the GHBP hydrophilic tail and 
      3'UTR sequence. 5'UTR L2 is encoded by a single exon located at least 27 kb 
      upstream of exon 2 and at least 12 kb upstream of the exon encoding 5'UTR L1. The 
      transcription start sites of UTR L2 were mapped and the 5' flanking region 
      sequenced. The exon and proximal promoter region are GC rich, and share a high 
      level of conservation with the equivalent exons in the sheep, bovine and human 
      GHR genes. A CCAAT motif and several putative Sp1 motifs are present, and there 
      is no TATA box. Homology between the mouse sequence and other species is limited 
      to a region of 450 bp upstream of the exon due to the insertion of a fragment of 
      a LINE-1 element upstream of the mouse L2 exon. Ribonuclease protection assays 
      were used to confirm that 5'UTR L2 is widely expressed in multiple tissues and is 
      the predominant form of transcript except in the liver during pregnancy, in which 
      5'UTR L1 is the major form.
FAU - Moffat, J G
AU  - Moffat JG
AD  - Department of Biology, University of California, Santa Cruz, California 95060, 
      USA.
FAU - Edens, A
AU  - Edens A
FAU - Talamantes, F
AU  - Talamantes F
LA  - eng
SI  - GENBANK/AF120480
SI  - GENBANK/AF120481
SI  - GENBANK/AF120482
SI  - GENBANK/AF120483
SI  - GENBANK/AF120484
SI  - GENBANK/AF120485
SI  - GENBANK/AF120486
SI  - GENBANK/AF120487
SI  - GENBANK/AF120488
SI  - GENBANK/AF120489
SI  - GENBANK/AH007734
GR  - CA71590/CA/NCI NIH HHS/United States
GR  - GM08132/GM/NIGMS NIH HHS/United States
GR  - HD14966/HD/NICHD NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Carrier Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Somatotropin)
RN  - 9007-49-2 (DNA)
RN  - W06KFL3RDT (somatotropin-binding protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/*genetics
MH  - Cloning, Molecular
MH  - DNA/chemistry/genetics/isolation & purification
MH  - Exons
MH  - Female
MH  - Gene Expression
MH  - Genes/genetics
MH  - Introns
MH  - Male
MH  - Mice
MH  - Mice, Inbred DBA
MH  - Molecular Sequence Data
MH  - Pregnancy
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Somatotropin/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Tissue Distribution
MH  - Transcription, Genetic
EDAT- 1999/07/30 00:00
MHDA- 1999/07/30 00:01
CRDT- 1999/07/30 00:00
PHST- 1999/07/30 00:00 [pubmed]
PHST- 1999/07/30 00:01 [medline]
PHST- 1999/07/30 00:00 [entrez]
AID - JME00787 [pii]
AID - 10.1677/jme.0.0230033 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 1999 Aug;23(1):33-44. doi: 10.1677/jme.0.0230033.