PMID- 10426121 OWN - NLM STAT- MEDLINE DCOM- 19990902 LR - 20191103 IS - 0921-9668 (Print) IS - 0921-9668 (Linking) VI - 52 IP - 4 DP - 1998 TI - Studies on the influence of temperature, relative humidity and microenvironment on the natural fermentation of African oil bean seeds to 'Ugba'. PG - 337-51 AB - Studies on the enhancement of the traditional production of 'Ugba' (a protein-rich fermented food) from African oil bean seeds were undertaken by fermenting the bean seeds at different temperatures, relative humidities (RH) and microenvironments. Fermentation was monitored by pH, texture, amino-nitrogen content and the viable cell count of the substrate. The 40 degrees C, 98% RH or the 130 microm high density polyethylene (HDPE) treatment increased the fermentation microflora from ca. 10(6) CFU/g to ca. 10(8) CFU/g with high initial changes in pH (5.8-ca. 7.9) and texture (2.0 kg/cm2 to between 1.4 kg/cm2 and 0.9 kg/cm2) in 24 hours. Products with amino-nitrogen contents of between 12.00 mg N/100 g dry matter and 14.00 mg N/100 g dry matter were obtained in 3 days. The cell count of the 30 degrees C, 80% RH or 70 microm treatment increased from 10(6) CFU/g to ca. 10(7) CFU/g and the pH increased from 5.8 to about 6.7 with a coincident decrease in the texture value from 2.0 kg/cm2 to about 1.7 kg/cm2 in 24 hours. Products with amino-nitrogen contents between 15.00 mg N/100 g dry matter and 19.2 mg N/100 g dry matter were obtained after 3 days. Changes in the fermentation indicators were not significant at p < or = 0.05 (pH and texture) and at p < or = 0.01 (amino-nitrogen) after 3 days for the 25 degrees C, 59.9% RH or 50 microm low density polyethylene (LDPE) treatment. Products of fermentation at the combined optimal conditions (80% RH, 35 degrees C, and 70 microm HDPE) compared very well with the traditionally fermented products in terms of pH, texture and amino-nitrogen content. FAU - Isu, N R AU - Isu NR AD - School of Biological Sciences, Abia State University, Uturu, Nigeria. FAU - Njoku, H O AU - Njoku HO LA - eng PT - Journal Article PL - Netherlands TA - Plant Foods Hum Nutr JT - Plant foods for human nutrition (Dordrecht, Netherlands) JID - 8803554 RN - N762921K75 (Nitrogen) SB - IM MH - *Environment MH - *Fabaceae MH - *Fermentation MH - Food MH - Humans MH - *Humidity MH - Hydrogen-Ion Concentration MH - Nitrogen/analysis MH - *Plants, Medicinal MH - Seeds MH - *Temperature EDAT- 1999/07/30 00:00 MHDA- 1999/07/30 00:01 CRDT- 1999/07/30 00:00 PHST- 1999/07/30 00:00 [pubmed] PHST- 1999/07/30 00:01 [medline] PHST- 1999/07/30 00:00 [entrez] AID - 10.1023/a:1008042726487 [doi] PST - ppublish SO - Plant Foods Hum Nutr. 1998;52(4):337-51. doi: 10.1023/a:1008042726487.