PMID- 10427043
OWN - NLM
STAT- MEDLINE
DCOM- 19990915
LR  - 20240109
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 65
IP  - 8
DP  - 1999 Aug
TI  - Optimization of terminal-restriction fragment length polymorphism analysis for 
      complex marine bacterioplankton communities and comparison with denaturing 
      gradient gel electrophoresis.
PG  - 3518-25
AB  - The potential of terminal-restriction fragment length polymorphism (T-RFLP) and 
      the detection of operational taxonomic units (OTUs) by capillary electrophoresis 
      (CE) to characterize marine bacterioplankton communities was compared with that 
      of denaturing gradient gel electrophoresis (DGGE). A protocol has been developed 
      to optimize the separation and detection of OTUs between 20 and 1, 632 bp by 
      using CE and laser-induced fluorescence detection. Additionally, we compared 
      T-RFLP fingerprinting to DGGE optimized for detection of less abundant OTUs. 
      Similar results were obtained with both fingerprinting techniques, although the 
      T-RFLP approach and CE detection of OTUs was more sensitive, as indicated by the 
      higher number of OTUs detected. We tested the T-RFLP fingerprinting technique on 
      complex marine bacterial communities by using the 16S rRNA gene and 16S rRNA as 
      templates for PCR. Samples from the Northern and Middle Adriatic Sea and from the 
      South and North Aegean Sea were compared. Distinct clusters were identifiable for 
      different sampling sites. Thus, this technique is useful for rapid evaluation of 
      the biogeographical distribution and relationships of bacterioplankton 
      communities.
FAU - Moeseneder, M M
AU  - Moeseneder MM
AD  - Department of Biological Oceanography, Netherlands Institute for Sea Research 
      (NIOZ), NL-1790 AB Den Burg, The Netherlands. markus@nioz.nl
FAU - Arrieta, J M
AU  - Arrieta JM
FAU - Muyzer, G
AU  - Muyzer G
FAU - Winter, C
AU  - Winter C
FAU - Herndl, G J
AU  - Herndl GJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - Bacteria/classification/*genetics
MH  - Bacteriological Techniques
MH  - Base Sequence
MH  - DNA Fingerprinting
MH  - DNA Primers/genetics
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - Ecosystem
MH  - Electrophoresis, Agar Gel/methods
MH  - Electrophoresis, Capillary/methods
MH  - Evaluation Studies as Topic
MH  - Plankton/classification/*genetics
MH  - *Polymorphism, Restriction Fragment Length
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Water Microbiology
PMC - PMC91528
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
PMCR- 1999/08/01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
PHST- 1999/08/01 00:00 [pmc-release]
AID - 0524 [pii]
AID - 10.1128/AEM.65.8.3518-3525.1999 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1999 Aug;65(8):3518-25. doi: 
      10.1128/AEM.65.8.3518-3525.1999.