PMID- 10427073 OWN - NLM STAT- MEDLINE DCOM- 19990915 LR - 20240109 IS - 0099-2240 (Print) IS - 1098-5336 (Electronic) IS - 0099-2240 (Linking) VI - 65 IP - 8 DP - 1999 Aug TI - Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. PG - 3721-6 AB - Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes was used to investigate the phylogenetic composition of bacterioplankton communities in several freshwater and marine samples. An average of about 50% of the cells were detected by probes for the domains Bacteria and Archaea, and of these, about half could be identified at the subdomain level with a set of group-specific probes. Beta subclass proteobacteria constituted a dominant fraction in freshwater systems, accounting for 16% (range, 3 to 32%) of the cells, although they were essentially absent in the marine samples examined. Members of the Cytophaga-Flavobacterium cluster were the most abundant group detected in the marine systems, accounting for 18% (range, 2 to 72%) of the 4',6-diamidino-2-phenylindole (DAPI) counts, and they were also important in freshwater systems (7%, range 0 to 18%). Furthermore, members of the alpha and gamma subclasses of Proteobacteria as well as members of the Planctomycetales were detected in both freshwater and marine water in abundances <7%. FAU - Glockner, F O AU - Glockner FO AD - Max-Planck-Institut fur Marine Mikrobiologie, Bremen, Germany. FAU - Fuchs, B M AU - Fuchs BM FAU - Amann, R AU - Amann R LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Appl Environ Microbiol JT - Applied and environmental microbiology JID - 7605801 RN - 0 (DNA Probes) SB - IM MH - Animals MH - Archaea/classification/genetics/isolation & purification MH - Bacteria/classification/genetics/isolation & purification MH - Base Sequence MH - Colony Count, Microbial MH - Cytophaga/classification/genetics/isolation & purification MH - DNA Probes/genetics MH - Flavobacterium/classification/genetics/isolation & purification MH - Fresh Water/*microbiology MH - In Situ Hybridization, Fluorescence MH - Plankton/*genetics/*isolation & purification MH - Seawater/*microbiology PMC - PMC91558 EDAT- 1999/07/31 00:00 MHDA- 1999/07/31 00:01 PMCR- 1999/08/01 CRDT- 1999/07/31 00:00 PHST- 1999/07/31 00:00 [pubmed] PHST- 1999/07/31 00:01 [medline] PHST- 1999/07/31 00:00 [entrez] PHST- 1999/08/01 00:00 [pmc-release] AID - 0378 [pii] AID - 10.1128/AEM.65.8.3721-3726.1999 [doi] PST - ppublish SO - Appl Environ Microbiol. 1999 Aug;65(8):3721-6. doi: 10.1128/AEM.65.8.3721-3726.1999.