PMID- 10427120
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20190516
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 15
IP  - 3
DP  - 1999 Sep
TI  - Amplification of telomeric DNA directly correlates with metastatic potential of 
      human and murine cancers of various histological origin.
PG  - 423-9
AB  - Telomeres, repeated DNA sequences (T2AG3)n that guard the ends of chromosomes, 
      serve as a checkpoint for cell-cycle progression and regulate cell senescence and 
      apoptosis. Loss of the telomeric repeats promotes genomic instability, which is 
      the hallmark of most cancer cells. Whether this loss differs among tumor cells 
      with malignant potential is unknown and was the goal of this study. An all-human 
      telomeric DNA probe was used to perform fluorescence in situ hybridization (FISH) 
      and the telomeric signals in interphase nuclei were quantitated using a computer 
      software package. Southern blot analysis was carried out to measure terminal 
      restriction fragment length (TRFL) in multiple cancer cell lines, including 
      nonmetastatic and metastatic human breast, lung, prostate, colon, brain, and 
      renal carcinomas, as well as human and murine melanoma clones and somatic cell 
      hybrids. The metastatic capability of all cell lines, clones and somatic cell 
      hybrids was evaluated subsequent to orthotopic implantation into nude mice. FISH 
      preparations with telomeric DNA probes showed that the mean percent telomeric 
      area in the metastatic nuclei was significantly greater than their nonmetastatic 
      counterparts and Southern blotting in selected samples confirmed our findings. 
      These data suggest that amplification of telomeres is directly correlated with 
      invasive and metastatic potential of murine or human tumor cells.
FAU - Multani, A S
AU  - Multani AS
AD  - Department of Cancer Biology, The University of Texas M.D. Anderson Cancer 
      Center, Houston, TX 77030, USA.
FAU - Ozen, M
AU  - Ozen M
FAU - Sen, S
AU  - Sen S
FAU - Mandal, A K
AU  - Mandal AK
FAU - Price, J E
AU  - Price JE
FAU - Fan, D
AU  - Fan D
FAU - Radinsky, R
AU  - Radinsky R
FAU - Ali-Osman, F
AU  - Ali-Osman F
FAU - Von Eschenbach, A C
AU  - Von Eschenbach AC
FAU - Fidler, I J
AU  - Fidler IJ
FAU - Pathak, S
AU  - Pathak S
LA  - eng
GR  - RRO-4999-01/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cell Cycle/genetics
MH  - Cell Nucleus/physiology
MH  - DNA Probes
MH  - DNA, Neoplasm/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Mice
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - *Telomere
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - 10.3892/ijo.15.3.423 [doi]
PST - ppublish
SO  - Int J Oncol. 1999 Sep;15(3):423-9. doi: 10.3892/ijo.15.3.423.