PMID- 10427134
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20071115
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 15
IP  - 3
DP  - 1999 Sep
TI  - The mitogen activated protein kinase pathway is required for proliferation but 
      not invasion of human squamous cell carcinoma lines.
PG  - 519-23
AB  - Growth factor receptors of the tyrosine kinase family regulate proliferation and 
      migration of a variety of cell types. Binding of cognate ligands to these 
      receptors induces multiple cellular responses, including cell cycle progression 
      and motility in culture model systems. In stratified squamous epithelial cells, 
      these receptors include epidermal growth factor receptor (EGFR) that binds both 
      EGF and transforming growth factor alpha (TGFalpha), and c-met whose ligand is 
      hepatocyte growth factor/scatter factor (HGF/SF). Intracellular signaling via 
      these receptors occurs by several mechanisms, including activation of ras, 
      phosphatidylinositol 3-kinase (PI3K), and the mitogen activated protein kinase 
      (MAPK) pathways. Growth factor independence is a characteristic feature of 
      transformation in cancer cells. Previous studies have shown that human squamous 
      cell carcinoma (SCC) lines do not require EGF or TGFalpha for proliferation. We 
      show that while these cell lines expressed EGFR and c-met, stimulation with their 
      respective ligands did not induce proliferation but markedly increased invasion 
      of reconstituted basement membranes. However, EGFR kinase activity was required 
      for proliferation and EGF induced invasion by these cells. Signaling via ras, 
      PI3K, and MAPK was required for proliferation of SCC lines. However, inhibition 
      of ras and MAPK did not significantly reduce invasion by these cells nor 
      completely block stimulation of this activity by EGF and HGF. We concluded that 
      MAPK signaling was required for proliferation but not invasion of human SCC 
      lines.
FAU - Tsang, D K
AU  - Tsang DK
AD  - Center for Craniofacial Molecular Biology, University of Southern California, Los 
      Angeles, CA 90033, USA.
FAU - Crowe, D L
AU  - Crowe DL
LA  - eng
GR  - DE10966/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Receptors, Growth Factor)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Carcinoma, Squamous Cell/*enzymology/pathology
MH  - Cell Division/physiology
MH  - Humans
MH  - Neoplasm Invasiveness
MH  - Receptors, Growth Factor/*physiology
MH  - Signal Transduction/physiology
MH  - Tumor Cells, Cultured
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 1999 Sep;15(3):519-23.