PMID- 10428040
OWN - NLM
STAT- MEDLINE
DCOM- 19990813
LR  - 20191210
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 73
IP  - 2
DP  - 1999 Aug
TI  - Roles of thapsigargin-sensitive Ca2+ stores in the survival of developing 
      cultured neurons.
PG  - 457-65
AB  - The roles of the intracellular calcium pool involved in regulating the Ca2+ 
      profile and the neuronal survival rate during development were studied by using 
      thapsigargin (TG), a specific inhibitor of endoplasmic reticulum (ER) Ca2+-ATPase 
      in cultured cerebellar granule neurons. Measuring the neuronal [Ca2+]i directly 
      in the culture medium, we found a bell-shaped curve for [Ca2+]i versus cultured 
      days in cerebellar granule neurons maintained in medium containing serum and 25 
      mM K+. The progressive increase in [Ca2+]i of the immature granule neurons (1-4 
      days in vitro) was abolished by TG, which resulted in massive neuronal apoptosis. 
      When the [K+] was lowered from 25 to 5 mM, neither the progressively increasing 
      [Ca2+]i nor the survival of immature granule neurons was significantly changed 
      over 24-h incubation. Similarly, TG caused a dramatic decrease in the [Ca2+]i and 
      survival rate of these immature neurons when switched to 5 mM K+ medium. 
      Following maturation, the granule neurons became less sensitive to TG for both 
      [Ca2+]i and neuronal survival. However, TG can protect mature granule neurons 
      from the detrimental effect of switching to a 5 mM K+ serum-free medium by 
      decreasing [Ca2+]i to an even lower level than in the respective TG-free group. 
      Based on these findings, we propose that during the immature stage, TG-sensitive 
      ER Ca2+-ATPase plays a pivotal role in the progressive increase of [Ca2+]i, which 
      is essential for the growth and maturation of cultured granule neurons.
FAU - Yao, C J
AU  - Yao CJ
AD  - Institutes of Toxicology, College of Medicine, National Taiwan University, 
      Taipei, ROC.
FAU - Lin, C W
AU  - Lin CW
FAU - Lin-Shiau, S Y
AU  - Lin-Shiau SY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Blood Proteins)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Neuroprotective Agents)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - RWP5GA015D (Potassium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Blood Proteins/pharmacology
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cellular Senescence/drug effects
MH  - Cerebellum/cytology
MH  - Culture Media, Serum-Free/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Endoplasmic Reticulum/metabolism
MH  - Enzyme Inhibitors/*pharmacology
MH  - Neurons/*cytology/drug effects/*enzymology
MH  - Neuroprotective Agents/pharmacology
MH  - Potassium/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Thapsigargin/*pharmacology
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.0730457.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 Aug;73(2):457-65. doi: 10.1046/j.1471-4159.1999.0730457.x.