PMID- 10428834
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 32
DP  - 1999 Aug 6
TI  - Retinoid-dependent recruitment of a histone H1 displacement activity by retinoic 
      acid receptor.
PG  - 22563-8
AB  - Targeted recruitment of histone acetyltransferase (HAT) activities by 
      sequence-specific transcription factors, including the retinoic acid receptors 
      (RARs) and retinoid X receptors (RXRs), has been proposed to lead to 
      destabilization of nucleosomal cores by acetylation of core histones. However, 
      biochemical evidence indicates that destabilization and depletion of linker H1 
      histones must also occur at the promoter regions of actively transcribing genes. 
      Mechanisms by which nuclear receptors and other transcription factors affect the 
      removal of histone H1 from transcriptionally silent chromatin have not been 
      previously described. In this report, we show that RARs interact in a 
      ligand-dependent manner with HMG-I, which is known to displace histone H1 from 
      chromatin. We further show that HMG-I and a novel related protein, HMG-R, also 
      interact with other transcription factors. Using sense and antisense constructs 
      of HMG-I/R in transient transfection assays with a retinoid responsive reporter, 
      we also demonstrate that HMG-I/R is important for retinoid dependent 
      transcriptional activity of RAR. These findings suggest a step wise mechanism by 
      which RARs and other transcription factors can cause a targeted unfolding of 
      compact chromatin as a first step in transcriptional activation, which would then 
      be followed by recruitment of HAT activity and subsequent events.
FAU - Nagpal, S
AU  - Nagpal S
AD  - Retinoid Research, Allergan Inc., Irvine, California 92713, USA. 
      nagpal_sunil@allergan.com
FAU - Ghosn, C
AU  - Ghosn C
FAU - DiSepio, D
AU  - DiSepio D
FAU - Molina, Y
AU  - Molina Y
FAU - Sutter, M
AU  - Sutter M
FAU - Klein, E S
AU  - Klein ES
FAU - Chandraratna, R A
AU  - Chandraratna RA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chromatin)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (Histones)
RN  - 0 (Ligands)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Retinoids)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (CREBBP protein, human)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
SB  - IM
MH  - Acetylation
MH  - Acetyltransferases/metabolism
MH  - Amino Acid Sequence
MH  - CREB-Binding Protein
MH  - Chromatin/metabolism
MH  - High Mobility Group Proteins/*metabolism
MH  - Histone Acetyltransferases
MH  - Histones/*metabolism
MH  - Humans
MH  - Ligands
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Protein Binding
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - Receptors, Retinoic Acid/*metabolism
MH  - Retinoid X Receptors
MH  - Retinoids/*metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Trans-Activators/metabolism
MH  - Transcription Factors/metabolism
MH  - *Transcriptional Activation
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - S0021-9258(18)81577-0 [pii]
AID - 10.1074/jbc.274.32.22563 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 6;274(32):22563-8. doi: 10.1074/jbc.274.32.22563.