PMID- 10432497
OWN - NLM
STAT- MEDLINE
DCOM- 19991004
LR  - 20190831
IS  - 0097-0549 (Print)
IS  - 0097-0549 (Linking)
VI  - 29
IP  - 2
DP  - 1999 Mar-Apr
TI  - Selective suppression of forward and recurrent "rapid" inhibition by local 
      application of picrotoxin in area CA1 of rat hippocampal slices.
PG  - 117-23
AB  - Living slices of rat hippocampus were used to study the possibility of 
      suppressing forward and recurrent "rapid" inhibition in area CA1 by local 
      application of picrotoxin, an antagonist of ionotropic gamma-aminobutyric acid 
      (GABAA) receptors. Application of picrotoxin to the apical dendrites increased 
      the duration of focal potentials recorded in the radial layer (143.0 +/- 7.5%, n 
      = 5; here and subsequently, results are presented as mean +/- error of the mean 
      and n is the number of experiments) but had no effect on the population peak in 
      the pyramidal layer (103.0 +/- 19.6%, n = 5). This is evidence for the existence 
      of suppression of direct, but not of recurrent inhibition. Application of 
      picrotoxin to the cell body layer, on the other hand, significantly increased the 
      population peak (654.5 +/- 245.1%, n = 4) and provoked convulsive activity in 
      neurons, demonstrating suppression of recurrent inhibition. Local application of 
      picrotoxin was further used to study the question of how completely antagonists 
      of glutamate ionotropic receptors sensitive to 
      alpha-amino-3-hydroxy-S-methyl-4-isoxazole propionic acid (AMPA) suppress 
      inhibition in solutions with low magnesium contents. This question is important 
      for interpreting experimental data obtained from measurements of the components 
      of excitatory postsynaptic potentials (EPSP), which depend on activation of 
      ionotropic glutamate receptors sensitive to N-methyl-d-aspartic acid (NMDA). A 
      number of studies have suggested that even at low concentrations, AMPA receptor 
      antagonists suppress forward inhibition to such an extent that it has no 
      significant effect on measurements of the NMDA component of EPSP. Our data do not 
      contradict this suggestion.
FAU - Bayazitov, I T
AU  - Bayazitov IT
AD  - Bashkir State University, Ufa, Russia.
FAU - Kleshchevnikov, A M
AU  - Kleshchevnikov AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurosci Behav Physiol
JT  - Neuroscience and behavioral physiology
JID - 0330471
RN  - 0 (GABA Antagonists)
RN  - 0 (GABA-A Receptor Antagonists)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 124-87-8 (Picrotoxin)
SB  - IM
MH  - Animals
MH  - Dendrites/drug effects
MH  - Electric Stimulation
MH  - Evoked Potentials/drug effects
MH  - Excitatory Postsynaptic Potentials/drug effects
MH  - GABA Antagonists/*pharmacology
MH  - GABA-A Receptor Antagonists
MH  - Hippocampus/*drug effects
MH  - In Vitro Techniques
MH  - Male
MH  - Neuronal Plasticity/drug effects
MH  - Picrotoxin/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, AMPA/antagonists & inhibitors
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
EDAT- 1999/08/05 00:00
MHDA- 1999/08/05 00:01
CRDT- 1999/08/05 00:00
PHST- 1999/08/05 00:00 [pubmed]
PHST- 1999/08/05 00:01 [medline]
PHST- 1999/08/05 00:00 [entrez]
AID - 10.1007/BF02465314 [doi]
PST - ppublish
SO  - Neurosci Behav Physiol. 1999 Mar-Apr;29(2):117-23. doi: 10.1007/BF02465314.