PMID- 10432590
OWN - NLM
STAT- MEDLINE
DCOM- 19991005
LR  - 20190915
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 87
IP  - 1
DP  - 1999 Jul
TI  - Viscosinamide, a new cyclic depsipeptide with surfactant and antifungal 
      properties produced by Pseudomonas fluorescens DR54.
PG  - 80-90
AB  - Pseudomonas fluorescens DR54 showed antagonistic properties against plant 
      pathogenic Pythium ultimum and Rhizoctonia solani both in vitro and in planta. 
      Antifungal activity was extractable from spent growth media, and fractionation by 
      semi-preparative HPLC resulted in isolation of an active compound, which was 
      identified as a new bacterial cyclic lipodepsipeptide, viscosinamide, using 1D 
      and 2D 1H-, 13C-NMR and mass spectrometry. The new antibiotic has biosurfactant 
      properties but differs from the known biosurfactant, viscosin, by containing 
      glutamine rather than glutamate at the amino acid position 2 (AA2). No viscosin 
      production was observed, however, when Ps. fluorescens DR54 was cultured in media 
      enriched with glutamate. In vitro tests showed that purified viscosinamide also 
      reduced fungal growth and aerial mycelium development of both P. ultimum and R. 
      solani. Viscosinamide production by Ps. fluorescens DR54 was tightly coupled to 
      cell proliferation in the batch cultures, as the viscosinamide produced per cell 
      mass unit approached a constant value. In batch cultures with variable initial C, 
      N or P nutrient levels, there were no indications of elevated viscosinamide 
      production during starvation or maintenance of the cultures in stationary phase. 
      Analysis of cellular fractions and spent growth media showed that a major 
      fraction of the viscosinamide produced remained bound to the cell membrane of Ps. 
      fluorescens DR54. The isolation, determination of structure and production 
      characteristics of the new compound with both biosurfactant and antibiotic 
      properties have promising perspectives for the application of Ps. fluorescens 
      DR54 in biological control.
FAU - Nielsen, T H
AU  - Nielsen TH
AD  - Department of Ecology, Royal Veterinary and Agricultural University, 
      Frederiksberg, Denmark. thn@kvl.dk
FAU - Christophersen, C
AU  - Christophersen C
FAU - Anthoni, U
AU  - Anthoni U
FAU - Sorensen, J
AU  - Sorensen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Antifungal Agents)
RN  - 0 (Culture Media)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (Surface-Active Agents)
RN  - 0 (viscosinamide)
SB  - IM
MH  - Antifungal Agents/*biosynthesis/chemistry/*pharmacology
MH  - Chromatography, High Pressure Liquid
MH  - Culture Media
MH  - Magnetic Resonance Spectroscopy
MH  - Mass Spectrometry
MH  - Peptides, Cyclic/*biosynthesis/chemistry/*pharmacology
MH  - Pseudomonas fluorescens/growth & development/*metabolism
MH  - Pythium/*drug effects/growth & development
MH  - Rhizoctonia/*drug effects/growth & development
MH  - Surface-Active Agents/chemistry/pharmacology
EDAT- 1999/08/05 00:00
MHDA- 1999/08/05 00:01
CRDT- 1999/08/05 00:00
PHST- 1999/08/05 00:00 [pubmed]
PHST- 1999/08/05 00:01 [medline]
PHST- 1999/08/05 00:00 [entrez]
AID - 10.1046/j.1365-2672.1999.00798.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 1999 Jul;87(1):80-90. doi: 10.1046/j.1365-2672.1999.00798.x.