PMID- 10433361
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20081121
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 19
IP  - 6
DP  - 1999 Jun
TI  - Kinetics of cytokine production in the cornea and trigeminal ganglion of C57BL/6 
      mice after corneal HSV-1 infection.
PG  - 609-15
AB  - To investigate the role of cytokines in the pathogenesis of acute herpetic 
      keratitis (HK), we examined the kinetics of cytokine expression in the corneas 
      and the trigeminal ganglia (TG) of C57BL/6Cr (B6) mice after herpes simplex virus 
      type 1 (HSV-1) infection and observed the influence of the targeted disruption of 
      interferon-gamma (IFN-gamma) gene on the clinical course of HK and/or viral 
      clearance. Following corneal infection with HSV-1 Amakata strain, all corneas 
      developed a typical dendritic keratitis. Quantitative analysis using 
      enzyme-linked immunosorbent assay (ELISA) revealed that the expression of 
      interleukin-1alpha (IL-1alpha), IL-5, IL-6, and IFN-gamma in corneas and TGs 
      significantly elevated immediately after infection, peaked between days 2 and 7 
      postinfection (p.i.), and then diminished. One exception was IFN-gamma, whose 
      expression significantly persisted in the TGs until day 30 p.i. An additional 
      experiment using IFN-gamma-/- (gko) mice revealed that there was no significant 
      difference in the peak level of viral replication in corneas and TGs between gko 
      and B6 mice, although gko mice showed a significant delay of virus clearance in 
      both corneas and TGs (p < 0.005) and higher mortality rate than B6 mice after 
      HSV-1 infection (p < 0.01). These data suggest that the production of 
      proinflammatory cytokines closely correlates with the pathogenesis of HK, and 
      that IFN-gamma plays an important role in enhancing viral clearance from the 
      cornea and TG.
FAU - He, J
AU  - He J
AD  - Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
FAU - Ichimura, H
AU  - Ichimura H
FAU - Iida, T
AU  - Iida T
FAU - Minami, M
AU  - Minami M
FAU - Kobayashi, K
AU  - Kobayashi K
FAU - Kita, M
AU  - Kita M
FAU - Sotozono, C
AU  - Sotozono C
FAU - Tagawa, Y I
AU  - Tagawa YI
FAU - Iwakura, Y
AU  - Iwakura Y
FAU - Imanishi, J
AU  - Imanishi J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Cytokines)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cornea/*metabolism/virology
MH  - Corneal Diseases/*metabolism/virology
MH  - Cytokines/*biosynthesis
MH  - Epithelial Cells/metabolism/virology
MH  - Herpes Simplex/*metabolism
MH  - Interferon-gamma/genetics
MH  - Kinetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Trigeminal Ganglion/*metabolism/virology
MH  - Virus Replication
EDAT- 1999/08/05 00:00
MHDA- 1999/08/05 00:01
CRDT- 1999/08/05 00:00
PHST- 1999/08/05 00:00 [pubmed]
PHST- 1999/08/05 00:01 [medline]
PHST- 1999/08/05 00:00 [entrez]
AID - 10.1089/107999099313749 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 1999 Jun;19(6):609-15. doi: 10.1089/107999099313749.