PMID- 10433817 OWN - NLM STAT- MEDLINE DCOM- 19990924 LR - 20220310 IS - 1044-7431 (Print) IS - 1044-7431 (Linking) VI - 14 IP - 1 DP - 1999 Jul TI - Excess target-derived brain-derived neurotrophic factor preserves the transient uncrossed retinal projection to the superior colliculus. PG - 52-65 AB - During early postnatal development, a widespread ipsilateral projection to the superior colliculus is secondarily restricted to a small topographically defined region by elimination of ipsilaterally projecting retinal ganglion cells. Brain-derived neurotrophic factor (BDNF) has been proposed as the target-derived neurotrophic factor for retinal ganglion cells in several studies. Here we investigated the long-term effects of excess BDNF in the retinal ganglion cell target on naturally occurring retinal ganglion cell (RGC) elimination and on the restriction of the ipsilateral projection. To this end, sustained overexpression of BDNF was achieved in the postnatal superior colliculus using an adenoviral vector. While the total number of retinal ganglion cells in the adenovirus-BDNF treated animals was unchanged, a much higher proportion of RGCs retained a projection to the ipsilateral superior colliculus. We conclude that an excess of target-derived BDNF does not reduce the net amount of naturally occurring cell death in the retino-collicular system, but prevents the negative selection of retinal ganglion cells making inappropriate topographic connections. CI - Copyright 1999 Academic Press. FAU - Isenmann, S AU - Isenmann S AD - Department of Neurology, University Hospital, Tubingen, D-72076, Germany. FAU - Cellerino, A AU - Cellerino A FAU - Gravel, C AU - Gravel C FAU - Bahr, M AU - Bahr M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Mol Cell Neurosci JT - Molecular and cellular neurosciences JID - 9100095 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Recombinant Proteins) RN - 0 (Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate) RN - EC 3.2.1.23 (beta-Galactosidase) SB - IM MH - Adenoviruses, Human MH - Animals MH - Axonal Transport MH - Brain-Derived Neurotrophic Factor/biosynthesis/genetics/*physiology MH - Functional Laterality MH - Gene Transfer Techniques MH - Genetic Vectors MH - Rats MH - Recombinant Proteins/biosynthesis MH - Retina/cytology/*physiology MH - Retinal Ganglion Cells/cytology/physiology MH - Sequence Deletion MH - Superior Colliculi/cytology/*physiology MH - Visual Pathways/cytology/*physiology MH - Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate MH - beta-Galactosidase/biosynthesis/genetics EDAT- 1999/08/06 00:00 MHDA- 1999/08/06 00:01 CRDT- 1999/08/06 00:00 PHST- 1999/08/06 00:00 [pubmed] PHST- 1999/08/06 00:01 [medline] PHST- 1999/08/06 00:00 [entrez] AID - S1044-7431(99)90763-7 [pii] AID - 10.1006/mcne.1999.0763 [doi] PST - ppublish SO - Mol Cell Neurosci. 1999 Jul;14(1):52-65. doi: 10.1006/mcne.1999.0763.