PMID- 10433927
OWN - NLM
STAT- MEDLINE
DCOM- 19990831
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 155
IP  - 2
DP  - 1999 Aug
TI  - Comparison of c-met expression in ovarian epithelial tumors and normal epithelia 
      of the female reproductive tract by quantitative laser scan microscopy.
PG  - 343-8
AB  - The transmembrane tyrosine kinase receptor c-met with its ligand, hepatocyte 
      growth factor/scatter factor (HGF/SF), acts as a mitogen, motogen, and morphogen 
      in many normal epithelia. HGF/SF-met signaling has also been implicated in 
      neoplastic progression and metastasis. In this study, immunofluorescence staining 
      and quantitative laser scanning confocal microscopy were used to measure c-met 
      expression in ovarian surface epithelial tumors from 17 oophorectomy specimens. 
      These specimens were from patients aged 25 to 81 (mean age, 52) and included 10 
      malignant tumors, 4 borderline tumors, and five benign tumors including a Brenner 
      tumor. For comparison, c-met expression was measured in normal tissues from the 
      same patients, including 4 ovarian surface epithelia, 4 fallopian tube epithelia, 
      2 endometria, and 3 endocervical epithelia, as well as 3 cases of endometriosis. 
      Relative pixel intensity values of c-met expression ranged from 0.4 in a normal 
      ovarian surface epithelium to 22.3 in a borderline serous tumor. Malignant tumors 
      (mean, 9.6) and borderline tumors (mean, 12.9) had higher average c-met 
      expression levels than normal tissues (mean, 3.6) and endometriosis (mean, 1.8). 
      The expression levels of benign tumors were intermediate (mean, 7.9). Among the 
      normal tissues, c-met expression in fallopian tubes (mean, 8.2; range, 3.4-12.9) 
      was higher than that of the other normal epithelia (mean, 1.6; range, 0.4-4.3). 
      In eight cases where both normal and malignant tissues were sampled, c-met 
      expression was significantly greater in malignant than in normal epithelia (P = 
      0.01). These findings indicate that c-met plays a role in the biology of the 
      normal tissues examined. They confirm that its expression increases in the 
      malignant progression of ovarian surface epithelial tumors, and suggest that 
      increases comparable to those in frankly malignant carcinomas have already been 
      reached in borderline lesions, ie, early in the neoplastic process.
FAU - Huntsman, D
AU  - Huntsman D
AD  - Department of Pathology, University of British Columbia, Vancouver, Canada.
FAU - Resau, J H
AU  - Resau JH
FAU - Klineberg, E
AU  - Klineberg E
FAU - Auersperg, N
AU  - Auersperg N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Adenocarcinoma/*genetics/*metabolism/pathology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cervix Uteri/anatomy & histology/metabolism
MH  - Endometriosis/metabolism/pathology
MH  - Epithelium/anatomy & histology/metabolism
MH  - Fallopian Tubes/anatomy & histology/metabolism
MH  - Female
MH  - Humans
MH  - *Microscopy, Confocal
MH  - Middle Aged
MH  - Ovarian Neoplasms/*genetics/*metabolism/pathology
MH  - Ovary/anatomy & histology/metabolism
MH  - Premenopause
MH  - Proto-Oncogene Proteins c-met/*metabolism
PMC - PMC1866871
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
PMCR- 2000/02/01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
PHST- 2000/02/01 00:00 [pmc-release]
AID - S0002-9440(10)65130-9 [pii]
AID - 1819 [pii]
AID - 10.1016/S0002-9440(10)65130-9 [doi]
PST - ppublish
SO  - Am J Pathol. 1999 Aug;155(2):343-8. doi: 10.1016/S0002-9440(10)65130-9.