PMID- 10434003
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 837
IP  - 1-2
DP  - 1999 Aug 7
TI  - Metalloprotease MP100: a synaptic protease in rat brain.
PG  - 193-202
AB  - Proteases are expressed widely throughout the nervous system and perform 
      essential functions. We have earlier characterized and cloned the metalloprotease 
      MP100, an enzyme originally described as a beta-amyloid precursor protein 
      (beta-APP) processing candidate. In the present study we describe the cellular 
      and subcellular localization of MP100 in rat brain. A punctuate intracellular 
      immunostaining in cortical, hippocampal and cerebellar neurons suggests its high 
      abundance in vesicular intracellular structures. The MP100 staining pattern 
      resembled that of the presynaptic protein synaptophysin. In gel filtration 
      chromatography of isolated rat brain synaptosomal membranes, MP100 
      co-fractionated with synaptophysin and beta-APP. Furthermore, pre-embedding 
      immunoelectron microscopy of the cerebellum revealed MP100 to be localized at 
      synaptic sites. All together, these data might indicate a role for MP100 in 
      functions such as proteolytic modification of synaptic proteins.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Huber, A B
AU  - Huber AB
AD  - Pharma Division, Preclinical CNS Research, F. Hoffmann-La Roche, Bldg. 69/452, 
      4002, Basel, Switzerland.
FAU - Brosamle, C
AU  - Brosamle C
FAU - Mechler, H
AU  - Mechler H
FAU - Huber, G
AU  - Huber G
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Synapsins)
RN  - 0 (Synaptophysin)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.- (NPEPPS protein, human)
SB  - IM
MH  - Animals
MH  - Brain/*enzymology
MH  - Cell Fractionation
MH  - Centrifugation, Zonal
MH  - Cerebellum/enzymology
MH  - Cerebral Cortex/enzymology
MH  - Hippocampus/enzymology
MH  - Immunohistochemistry
MH  - Intracellular Membranes/enzymology/ultrastructure
MH  - Metalloendopeptidases/*analysis
MH  - Microscopy, Immunoelectron
MH  - Neurons/enzymology/ultrastructure
MH  - Rats
MH  - Synapses/*enzymology/ultrastructure
MH  - Synapsins/analysis
MH  - Synaptic Membranes/enzymology/ultrastructure
MH  - Synaptophysin/analysis
MH  - Synaptosomes/enzymology/ultrastructure
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
AID - S0006-8993(99)01693-5 [pii]
AID - 10.1016/s0006-8993(99)01693-5 [doi]
PST - ppublish
SO  - Brain Res. 1999 Aug 7;837(1-2):193-202. doi: 10.1016/s0006-8993(99)01693-5.