PMID- 10435294
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20190822
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 123
IP  - 12
DP  - 1998 Dec
TI  - Production of CTC-containing porcine reference materials.
PG  - 2535-9
AB  - The European Commission (EC) established the Standards, Measurements and Testing 
      programme for the preparation of Reference Materials (RMs) as an aid to harmonise 
      testing for veterinary drug residues throughout the European Union (EU). The 
      production of chlortetracycline (CTC)-free and CTC-incurred pig tissues as 
      candidate RMs is described. High performance liquid chromatography (HPLC) with 
      fluorescence detection of CTC and 4-epi-CTC was used for all tissue analyses. A 
      pilot study revealed that incurred CTC residues were stable in pig kidney, liver 
      and muscle lyophilised powders during storage for 10 weeks at -70, -20 and +37 
      degrees C, obviating the need for addition of a stabiliser (thimerosal). In the 
      main study, 500 vials each of CTC-free and CTC-incurred kidney, liver and muscle 
      were produced. Target concentrations in the CTC-incurred lyophilised tissue 
      powders were 750-1500, 500-1000 and 300-600 micrograms kg-1 for kidney, liver and 
      muscle, respectively. Following lyophilisation, the mean +/- s concentrations of 
      CTC in the incurred positive RMs were 1,315 +/- 56.9, 765 +/- 35.3 and 378 +/- 
      16.8 micrograms kg-1 for kidney, liver and muscle respectively. Residual moisture 
      in the RMs ranged from 1.6 +/- 0.53% for muscle to 3.0 +/- 0.50% for liver. 
      Between-vial homogeneity for incurred powders was determined for 20 vials of each 
      material, which had been removed at regular intervals during the filling process. 
      Relative standard deviations (RSDs) for kidney, liver and muscle were 4.3, 4.6 
      and 4.4% respectively, being within the interassay RSD of the method and 
      indicating that mixing was effective. Stability of powders stored at -18, 4, 20 
      and 37 degrees C was assessed over a period of 79 weeks. No measurable 
      degradation occurred over this time period at any of the storage temperatures. It 
      is concluded that these candidate RMs are homogenous, stable and are suitable for 
      certification.
FAU - McEvoy, J D
AU  - McEvoy JD
AD  - Department of Agriculture for Northern Ireland, Veterinary Sciences Division, 
      Stormont, Belfast, UK. John.McEvoy@dani.gov.uk
FAU - Ferguson, J P
AU  - Ferguson JP
FAU - Crooks, S R
AU  - Crooks SR
FAU - Kennedy, D G
AU  - Kennedy DG
FAU - van Ginkel, L A
AU  - van Ginkel LA
FAU - Maghuin-Rogister, G
AU  - Maghuin-Rogister G
FAU - Meyer, H H
AU  - Meyer HH
FAU - Pfaffl, M W
AU  - Pfaffl MW
FAU - Farrington, W H
AU  - Farrington WH
FAU - Juhel-Gaugain, M
AU  - Juhel-Gaugain M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Anti-Bacterial Agents)
RN  - WCK1KIQ23Q (Chlortetracycline)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*standards
MH  - Chlortetracycline/*standards
MH  - Chromatography, High Pressure Liquid
MH  - Drug Residues/*analysis
MH  - European Union
MH  - Kidney/chemistry
MH  - Liver/chemistry
MH  - Meat/*analysis
MH  - Muscle, Skeletal/chemistry
MH  - Reference Standards
MH  - Swine
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
AID - 10.1039/a805199b [doi]
PST - ppublish
SO  - Analyst. 1998 Dec;123(12):2535-9. doi: 10.1039/a805199b.