PMID- 10438566
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20091119
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 251
IP  - 1
DP  - 1999 Aug 25
TI  - Hepatocyte growth factor/scatter factor promotes a switch from E- to N-cadherin 
      in chick embryo epiblast cells.
PG  - 3-15
AB  - Epiblast cells downregulate E-cadherin and upregulate N-cadherin as they ingress 
      through the primitive streak and when placed in culture. The factors that promote 
      the alteration in cadherin expression during gastrulation are unknown. The 
      effects of hepatocyte growth factor/scatter factor (HGF/SF) on cadherin 
      expression were tested in cultures of prestreak epiblast cells. HGF/SF decreased 
      the expression of E-cadherin and increased the percentage of cells with 
      N-cadherin and sarcomeric myosin. Cells with N-cadherin but not E-cadherin 
      differentiated into skeletal muscle. HGF/SF also stimulated proliferation and the 
      formation of cellular aggregates. Sensitivity to HGF/SF in vitro depended on the 
      original position of cells within the epiblast. More cells from the lateral 
      epiblast switched cadherins and proliferated in response to HGF/SF than medial 
      epiblast cells. HGF/SF may affect gastrulation by altering cadherin expression, 
      modulating cell adhesion, and stimulating proliferation within the epiblast.
CI  - Copyright 1999 Academic Press.
FAU - DeLuca, S M
AU  - DeLuca SM
AD  - Department of Anatomy, Philadelphia College of Osteopathic Medicine, 
      Philadelphia, Pennsylvania 19131, USA.
FAU - Gerhart, J
AU  - Gerhart J
FAU - Cochran, E
AU  - Cochran E
FAU - Simak, E
AU  - Simak E
FAU - Blitz, J
AU  - Blitz J
FAU - Mattiacci-Paessler, M
AU  - Mattiacci-Paessler M
FAU - Knudsen, K
AU  - Knudsen K
FAU - George-Weinstein, M
AU  - George-Weinstein M
LA  - eng
GR  - HD33086/HD/NICHD NIH HHS/United States
GR  - HD36650/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Cadherins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - Animals
MH  - Cadherins/*metabolism
MH  - Cell Aggregation/drug effects
MH  - Cell Count/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Dose-Response Relationship, Drug
MH  - Fluorescent Antibody Technique
MH  - Gastrula/*cytology/drug effects/*metabolism
MH  - Gene Expression Regulation, Developmental/*drug effects
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Muscle, Skeletal/cytology/drug effects/embryology/metabolism
MH  - Myosins/metabolism
MH  - Neurons/cytology/drug effects/metabolism
MH  - Proto-Oncogene Proteins c-met/metabolism
MH  - Time Factors
EDAT- 1999/08/10 00:00
MHDA- 1999/08/10 00:01
CRDT- 1999/08/10 00:00
PHST- 1999/08/10 00:00 [pubmed]
PHST- 1999/08/10 00:01 [medline]
PHST- 1999/08/10 00:00 [entrez]
AID - S0014-4827(99)94577-9 [pii]
AID - 10.1006/excr.1999.4577 [doi]
PST - ppublish
SO  - Exp Cell Res. 1999 Aug 25;251(1):3-15. doi: 10.1006/excr.1999.4577.