PMID- 10438961
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20081121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 4
DP  - 1999 Aug 15
TI  - Differential monocyte chemoattractant protein-1 and chemokine receptor 2 
      expression by murine lung fibroblasts derived from Th1- and Th2-type pulmonary 
      granuloma models.
PG  - 2193-201
AB  - Recent studies suggest that monocyte chemoattractant protein-1 (MCP-1) is 
      involved in fibrosis through the regulation of profibrotic cytokine generation 
      and matrix deposition. Changes in MCP-1, C-C chemokine receptor 2 (CCR2), 
      procollagen I and III, and TGF beta were examined in fibroblasts cultured from 
      normal lung and from nonfibrotic (i.e., Th1-type) and fibrotic (i.e., Th2-type) 
      pulmonary granulomas. Th2-type fibroblasts generated 2-fold more MCP-1 than 
      similar numbers of Th1-type or normal fibroblasts after 24 h in culture. Unlike 
      normal and Th1-type fibroblasts, Th2-type fibroblasts displayed CCR2 mRNA at 24 h 
      after IL-4 treatment. By flow cytometry, CCR2 was present on 40% of untreated 
      Th2-type fibroblasts, whereas CCR2 was present on <20% of normal and Th1-type 
      fibroblasts after similar treatment. IL-4 increased the number of normal 
      fibroblasts with cell-surface CCR2 but IFN-gamma-treatment of normal and Th2-type 
      fibroblasts significantly decreased the numbers of CCR2-positive cells in both 
      populations. Western blot analysis showed that total CCR2 protein expression was 
      markedly increased in untreated Th2-type fibroblasts compared with normal and 
      Th1-type fibroblasts. IL-4 treatment enhanced CCR2 protein in Th1- and Th2-type 
      fibroblasts whereas IFN-gamma treatment augmented CCR2 protein in normal and 
      Th1-type fibroblasts. All three fibroblast populations exhibited MCP-1-dependent 
      TGF-beta synthesis, but only normal and Th2-type fibroblasts showed a MCP-1 
      requirement for procollagen mRNA expression. Taken together, these findings 
      suggest that lung fibroblasts are altered in their expression of MCP-1, TGF-beta, 
      CCR2, and procollagen following their participation in pulmonary inflammatory 
      processes, and these changes may be important during fibrosis.
FAU - Hogaboam, C M
AU  - Hogaboam CM
AD  - Department of Pathology, Division of Pulmonary and Critical Care, University of 
      Michigan Medical School, Ann Arbor 48109, USA. hogaboam@path.med.umich.edu
FAU - Bone-Larson, C L
AU  - Bone-Larson CL
FAU - Lipinski, S
AU  - Lipinski S
FAU - Lukacs, N W
AU  - Lukacs NW
FAU - Chensue, S W
AU  - Chensue SW
FAU - Strieter, R M
AU  - Strieter RM
FAU - Kunkel, S L
AU  - Kunkel SL
LA  - eng
GR  - 1P50HL60289/HL/NHLBI NIH HHS/United States
GR  - HL35276/HL/NHLBI NIH HHS/United States
GR  - P01-HL31963/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Procollagen)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Transforming Growth Factor beta)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/physiology
MH  - Disease Models, Animal
MH  - Female
MH  - Fibroblasts/*metabolism
MH  - Flow Cytometry
MH  - Gene Expression Regulation/immunology
MH  - Granuloma, Respiratory Tract/*immunology/metabolism
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Lung/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Procollagen/biosynthesis/genetics
MH  - RNA, Messenger/drug effects/metabolism
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/*biosynthesis
MH  - Receptors, Cytokine/analysis/*biosynthesis/genetics
MH  - Th1 Cells/chemistry/*metabolism
MH  - Th2 Cells/chemistry/*metabolism
MH  - Transforming Growth Factor beta/biosynthesis
EDAT- 1999/08/10 00:00
MHDA- 1999/08/10 00:01
CRDT- 1999/08/10 00:00
PHST- 1999/08/10 00:00 [pubmed]
PHST- 1999/08/10 00:01 [medline]
PHST- 1999/08/10 00:00 [entrez]
AID - ji_v163n4p2193 [pii]
PST - ppublish
SO  - J Immunol. 1999 Aug 15;163(4):2193-201.