PMID- 10439339
OWN - NLM
STAT- MEDLINE
DCOM- 19990928
LR  - 20190921
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 19
IP  - 4
DP  - 1999 Jul-Aug
TI  - Local lymph node assay responses to paraphenylenediamine: intra- and 
      inter-laboratory evaluations.
PG  - 255-60
AB  - The murine local lymph node assay (LLNA) is a method for the prospective 
      identification of skin sensitizing chemicals. Proliferative responses induced in 
      lymph nodes draining the site of topical application of the test chemical are 
      measured and those chemicals that induce a stimulation index of three or more 
      compared with concurrent vehicle-treated controls are considered to have the 
      potential to cause skin sensitization. Dose-response data from the LLNA may be 
      used to derive an estimate of relative skin sensitizing potency, based upon 
      derivation of the concentration of chemical required to cause a stimulation index 
      of 3 (EC3 value) as calculated by linear interpolation. The purpose of the 
      present investigations was to examine the stability of LLNA responses and the 
      consistency of derived EC3 values induced by the contact allergen 
      paraphenylenediamine (PPD). Analyses were conducted once a month over a 4-month 
      period in each of two independent laboratories. In all assays, and in both 
      laboratories, PPD elicited a positive response. Although some minor differences 
      in responses between and within laboratories were observed, the derived EC3 
      values were generally very consistent. In Laboratory 1, EC3 values varied between 
      0.06 and 0.09% PPD, whereas in Laboratory 2 the range was 0.09-0.20%. These EC3 
      values are consistent with clinical experience of this material insofar as it is 
      a common and relatively potent cause of allergic contact dermatitis in humans. 
      Taken together, these data confirm the stability of LLNA responses both with time 
      and between laboratories and provide additional support for the use of derived 
      EC3 values in the assessment of relative skin sensitizing potency.
FAU - Warbrick, E V
AU  - Warbrick EV
AD  - Zeneca Central Toxicology Laboratory, Macclesfield, Cheshire, UK. 
      Vicky.Warbrick@CTL.ZENECA.com
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Lea, L J
AU  - Lea LJ
FAU - Basketter, D A
AU  - Basketter DA
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Allergens)
RN  - 0 (Phenylenediamines)
RN  - U770QIT64J (4-phenylenediamine)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animals
MH  - Cell Separation
MH  - Dermatitis, Contact/etiology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Laboratories/standards
MH  - Lymph Nodes/*drug effects/metabolism/pathology
MH  - Lymphocyte Activation/*drug effects
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Phenylenediamines/*toxicity
MH  - Predictive Value of Tests
MH  - Time Factors
MH  - Toxicity Tests
EDAT- 1999/08/10 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/08/10 10:00
PHST- 1999/08/10 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/08/10 10:00 [entrez]
AID - 10.1002/(SICI)1099-1263(199907/08)19:4<255::AID-JAT573>3.0.CO;2-S [pii]
AID - 10.1002/(sici)1099-1263(199907/08)19:4<255::aid-jat573>3.0.co;2-s [doi]
PST - ppublish
SO  - J Appl Toxicol. 1999 Jul-Aug;19(4):255-60. doi: 
      10.1002/(sici)1099-1263(199907/08)19:4<255::aid-jat573>3.0.co;2-s.