PMID- 10439340
OWN - NLM
STAT- MEDLINE
DCOM- 19990928
LR  - 20190921
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 19
IP  - 4
DP  - 1999 Jul-Aug
TI  - A comparison of statistical approaches to the derivation of EC3 values from local 
      lymph node assay dose responses.
PG  - 261-6
AB  - Effective risk assessment and management of allergic contact dermatitis require 
      three key factors: adequate hazard identification, measurement of the relative 
      potency of identified hazards and an understanding of the nature, extent and 
      duration of exposure. Suitable methods for hazard identification, such as the 
      murine local lymph node assay (LLNA) and the guinea-pig maximization test, are 
      well established and conditions of human exposure normally can be well 
      anticipated. Thus, the need is for a robust and quantitative method for the 
      estimation of relative skin sensitizing potency. One possible approach is via the 
      analysis of LLNA dose-response data. In the LLNA, contact allergens are defined 
      currently as those chemicals that cause a threefold or greater increase in lymph 
      node cell proliferative activity compared with concurrent vehicle-treated 
      controls. It is possible to estimate the concentration of a sensitizer required 
      to generate a threefold stimulation of proliferation in draining lymph nodes; 
      such a concentration is known as the EC3 value. Using a variety of statistical 
      approaches to derive EC3 values from LLNA dose-response data for 10 chemicals, it 
      has been demonstrated that simple linear interpolation between the values either 
      side of the threefold stimulation index provides a robust assessment of the EC3 
      value without the need for recourse to more sophisticated statistical techniques. 
      Provided that the appropriate concentrations of test chemical have been selected, 
      EC3 values obtained in this way are reproducible both within and between 
      laboratories and form the basis for examination of the utility of this approach 
      for the estimation of relative skin sensitizing potency.
FAU - Basketter, D A
AU  - Basketter DA
AD  - Safety and Environmental Assurance Centre, Unilever Research, Sharnbrook, UK.
FAU - Lea, L J
AU  - Lea LJ
FAU - Dickens, A
AU  - Dickens A
FAU - Briggs, D
AU  - Briggs D
FAU - Pate, I
AU  - Pate I
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animals
MH  - *Data Interpretation, Statistical
MH  - Dose-Response Relationship, Drug
MH  - Linear Models
MH  - Lymph Nodes/*drug effects/metabolism/pathology
MH  - Lymphocyte Activation/*drug effects
MH  - Mice
MH  - Mice, Inbred CBA
MH  - *Models, Statistical
MH  - Predictive Value of Tests
MH  - Toxicity Tests
EDAT- 1999/08/10 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/08/10 10:00
PHST- 1999/08/10 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/08/10 10:00 [entrez]
AID - 10.1002/(SICI)1099-1263(199907/08)19:4<261::AID-JAT572>3.0.CO;2-5 [pii]
AID - 10.1002/(sici)1099-1263(199907/08)19:4<261::aid-jat572>3.0.co;2-5 [doi]
PST - ppublish
SO  - J Appl Toxicol. 1999 Jul-Aug;19(4):261-6. doi: 
      10.1002/(sici)1099-1263(199907/08)19:4<261::aid-jat572>3.0.co;2-5.