PMID- 10440715
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20190905
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 412
IP  - 1
DP  - 1999 Sep 13
TI  - Mu-opioid and NMDA-type glutamate receptors are often colocalized in spiny 
      neurons within patches of the caudate-putamen nucleus.
PG  - 132-46
AB  - The patch compartments of the caudate-putamen nucleus (CPN) are enriched in 
      mu-opioid receptors (MORs) and have been recently implicated in reward-related 
      behaviors. This function has been established more clearly in the nucleus 
      accumbens, where physiological and anatomical studies show reward-associated 
      interactions involving MORs and N-methyl-D-aspartate-type glutamate receptors 
      (NMDARs). We examined the immunolabeling for MOR and NMDAR subunit NR1 in patches 
      of the rat CPN to determine the potential relevance of dual activation of the 
      respective receptors. Electron microscopy showed the presence of MOR and/or NR1 
      immunoreactivity (IR) in many perikarya, dendrites, and spines and in 
      morphologically heterogeneous axon terminals. In each 1,000-microm(2) area, the 
      dually labeled dendrites and spines constituted 65% (37/57) and 37% (9/25) of the 
      total NR1-labeled and 34% (37/109) and 13% (9/71) of the total MOR-labeled 
      dendritic profiles. Dually labeled spines received asymmetric excitatory-type 
      synapses from terminals, which were generally unlabeled, but also occasionally 
      contained MOR and/or NR1. The asymmetric synapses comprised the majority (81%) of 
      the total 263 synaptic contacts between MOR- and NR1-labeled neuronal profiles. 
      In dendrites and spines, MOR-IR was localized mainly along nonsynaptic plasma 
      membranes, whereas NR1-IR was more often associated with asymmetric postsynaptic 
      densities and cytoplasmic organelles. In contrast to dendrites, 6% (1.3/22) of 
      NR1-IR and 4% (1.3/33) of MOR-IR axon terminals were dually labeled in each 
      1,000-microm(2) area. Most singly or dually labeled terminals formed asymmetric 
      synapses with MOR- or NR1-labeled spines. Our results suggest that opioids acting 
      through MOR and excitatory neurotransmitters through NMDAR dually regulate the 
      output of single spiny neurons and some of their excitatory afferents in the CPN.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Wang, H
AU  - Wang H
AD  - Division of Neurobiology, Department of Neurology and Neuroscience, Joan and 
      Sanford I. Weill Medical College of Cornell University, New York, New York 10021, 
      USA. hwang@mail.med.cornell.edu
FAU - Gracy, K N
AU  - Gracy KN
FAU - Pickel, V M
AU  - Pickel VM
LA  - eng
GR  - DA046000/DA/NIDA NIH HHS/United States
GR  - MH00078/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Receptors, Opioid, mu)
SB  - IM
MH  - Animals
MH  - Axons/metabolism/ultrastructure
MH  - Caudate Nucleus/anatomy & histology/cytology/*metabolism
MH  - Cell Count
MH  - Dendrites/metabolism/ultrastructure
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Electron
MH  - Neurons/*metabolism/ultrastructure
MH  - Putamen/anatomy & histology/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Receptors, Opioid, mu/*metabolism
EDAT- 1999/08/10 00:00
MHDA- 1999/08/10 00:01
CRDT- 1999/08/10 00:00
PHST- 1999/08/10 00:00 [pubmed]
PHST- 1999/08/10 00:01 [medline]
PHST- 1999/08/10 00:00 [entrez]
AID - 10.1002/(SICI)1096-9861(19990913)412:1<132::AID-CNE10>3.0.CO;2-B [pii]
AID - 10.1002/(sici)1096-9861(19990913)412:1<132::aid-cne10>3.0.co;2-b [doi]
PST - ppublish
SO  - J Comp Neurol. 1999 Sep 13;412(1):132-46. doi: 
      10.1002/(sici)1096-9861(19990913)412:1<132::aid-cne10>3.0.co;2-b.