PMID- 10440870
OWN - NLM
STAT- MEDLINE
DCOM- 19990910
LR  - 20190915
IS  - 0270-4137 (Print)
IS  - 0270-4137 (Linking)
VI  - 41
IP  - 1
DP  - 1999 Sep 15
TI  - Molecular cytogenetic studies of a serially transplanted primary prostatic 
      carcinoma xenograft (CWR22) and four relapsed tumors.
PG  - 7-11
AB  - BACKGROUND: Established cell lines or xenografts from prostatic carcinoma have 
      been infrequently studied cytogenetically. CWR22 and CWR22-R are xenografts that 
      are unique in offering one strongly androgen-dependent and several relapsed 
      strains of a human prostate cancer that can be investigated in the laboratory. We 
      report on the cytogenetic characterization of the hormone-dependent CWR22, and 
      the relapsed CWR22-R serially transplanted xenografts, in our laboratory. 
      METHODS: We utilized a suspension harvest of the xenograft tissue to optimize our 
      yield for metaphase chromosome studies and analyzed the hormone-dependent CWR22 
      and four relapsed CWR22-R xenografts. These studies were accomplished using 
      standard G-banded analysis and fluorescence in situ hybridization (FISH). A 
      variety of DNA probes including alpha-satellite DNA probes, and chromosomal 
      libraries, were utilized for the FISH analysis. RESULTS: Utilizing both standard 
      cytogenetic analysis and FISH studies we have more precisely defined the CWR22 
      xenograft: 49,XY,+i(1)(q10),-2, der(4)t(2;4)(p21;q33), +7,+8,+12[7]/50,XY,idem, 
      +der(2)t(2;4)(p21;q33)del(2)(q13q33)[13]. Four relapsed xenografts, CWR22R-2152, 
      CWR22R-2524, CWR22R-2274, and CWR22R-2272 were also studied. Each of these lines 
      demonstrated a different karyotype. CONCLUSIONS: The CWR22 karyotype offers the 
      simplest reported karyotype for a prostate cancer tissue culture cell line or 
      xenograft; this makes CWR22 an attractive candidate for studies of genetic 
      changes associated with the relapse of prostate cancer treated with androgen 
      withdrawal. Four separate, serially transplanted, relapsed CWR22-R xenografts 
      were detected, each with a separate karyotype.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Kochera, M
AU  - Kochera M
AD  - Department of Genetics and Center for Human Genetics, Case Western Reserve 
      University School of Medicine and University Hospitals of Cleveland, Cleveland, 
      Ohio, USA.
FAU - Depinet, T W
AU  - Depinet TW
FAU - Pretlow, T P
AU  - Pretlow TP
FAU - Giaconia, J M
AU  - Giaconia JM
FAU - Edgehouse, N L
AU  - Edgehouse NL
FAU - Pretlow, T G
AU  - Pretlow TG
FAU - Schwartz, S
AU  - Schwartz S
LA  - eng
GR  - CA43703/CA/NCI NIH HHS/United States
GR  - CA57179/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - 0 (Androgens)
SB  - IM
MH  - Androgens/physiology
MH  - Animals
MH  - Chromosome Aberrations/genetics
MH  - Chromosome Banding
MH  - Chromosomes, Human/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Mice
MH  - Mosaicism/genetics
MH  - Neoplasm Recurrence, Local
MH  - *Neoplasm Transplantation
MH  - Orchiectomy
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - *Transplantation, Heterologous
MH  - Tumor Cells, Cultured
EDAT- 1999/08/10 00:00
MHDA- 1999/08/10 00:01
CRDT- 1999/08/10 00:00
PHST- 1999/08/10 00:00 [pubmed]
PHST- 1999/08/10 00:01 [medline]
PHST- 1999/08/10 00:00 [entrez]
AID - 10.1002/(SICI)1097-0045(19990915)41:1<7::AID-PROS2>3.0.CO;2-1 [pii]
AID - 10.1002/(sici)1097-0045(19990915)41:1<7::aid-pros2>3.0.co;2-1 [doi]
PST - ppublish
SO  - Prostate. 1999 Sep 15;41(1):7-11. doi: 
      10.1002/(sici)1097-0045(19990915)41:1<7::aid-pros2>3.0.co;2-1.