PMID- 10440875
OWN - NLM
STAT- MEDLINE
DCOM- 19990910
LR  - 20190915
IS  - 0270-4137 (Print)
IS  - 0270-4137 (Linking)
VI  - 41
IP  - 1
DP  - 1999 Sep 15
TI  - Isochromosome 8q formation is associated with 8p loss of heterozygosity in a 
      prostate cancer cell line.
PG  - 49-57
AB  - BACKGROUND: In advanced prostate cancer, loss of chromosomal regions on 8p is 
      frequently associated with gain of 8q. We studied the gross chromosomal 
      abnormalities associated with 8p loss of heterozygosity (LOH) in the prostate 
      tumor cell line 1542 CP3Tx. The cell line was previously established from a 
      primary prostatic adenocarcinoma by immortalization with a recombinant retrovirus 
      carrying the E6 and E7 genes of human papilloma virus type 16. Allelotyping 
      studies demonstrated LOH at multiple markers on 8p. METHODS: To investigate the 
      relationship of 8p LOH to gross chromosomal rearrangements, and to screen for 
      other genetic abnormalities in 1542 CP3Tx, we used comparative genomic 
      hybridization (CGH), conventional karyotyping, fluorescence in situ hybridization 
      (FISH), and allelotyping. RESULTS: CGH revealed loss of the entire 8p arm, 
      associated with gain of the entire 8q arm. Other abnormalities included 
      chromosome 4 loss and chromosome 11 gain. The karyotype showed an isochromosome 
      (8q), monosomy 4, and trisomy 11. FISH and allelotyping confirmed and extended 
      these results. CONCLUSIONS: These results demonstrate that i(8q) formation is a 
      mechanism for associated 8p loss and 8q gain in prostate cancer. Furthermore, the 
      small number of chromosomal abnormalities in this cell line indicates that 
      immortalization of low-passage cultures with viral oncogenes provides a method 
      for obtaining cell lines for studying genetic abnormalities in prostate cancer.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Virgin, J B
AU  - Virgin JB
AD  - Barbara Ann Karmanos Cancer Institute, Wayne State University, Detroit, Michigan, 
      USA. jvirgin@med.wayne.edu
FAU - Hurley, P M
AU  - Hurley PM
FAU - Nahhas, F A
AU  - Nahhas FA
FAU - Bebchuk, K G
AU  - Bebchuk KG
FAU - Mohamed, A N
AU  - Mohamed AN
FAU - Sakr, W A
AU  - Sakr WA
FAU - Bright, R K
AU  - Bright RK
FAU - Cher, M L
AU  - Cher ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Alleles
MH  - Chromosome Banding
MH  - Chromosome Painting
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - DNA Probes/genetics
MH  - Genetic Markers
MH  - Humans
MH  - Karyotyping
MH  - Loss of Heterozygosity/*genetics
MH  - Male
MH  - Prostatic Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1999/08/10 00:00
MHDA- 1999/08/10 00:01
CRDT- 1999/08/10 00:00
PHST- 1999/08/10 00:00 [pubmed]
PHST- 1999/08/10 00:01 [medline]
PHST- 1999/08/10 00:00 [entrez]
AID - 10.1002/(SICI)1097-0045(19990915)41:1<49::AID-PROS7>3.0.CO;2-8 [pii]
AID - 10.1002/(sici)1097-0045(19990915)41:1<49::aid-pros7>3.0.co;2-8 [doi]
PST - ppublish
SO  - Prostate. 1999 Sep 15;41(1):49-57. doi: 
      10.1002/(sici)1097-0045(19990915)41:1<49::aid-pros7>3.0.co;2-8.