PMID- 10440906
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20131121
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 57
IP  - 4
DP  - 1999 Aug 15
TI  - AMPA receptor protein expression and function in astrocytes cultured from 
      hippocampus.
PG  - 557-71
AB  - Glutamate receptors guide the proliferation, migration, and differentiation of 
      glial cells. Here, we characterize AMPA 
      (alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid) and NMDA receptor 
      protein expression and function and mRNA expression in hippocampal glial 
      cultures. By immunocytochemistry, GluR2 (the subunit that limits the Ca(2+) 
      permeability of AMPA receptors) exhibited prominent labeling in hippocampal glial 
      cultures. Double-labeling of GluR2 with GFAP and with A2B5 revealed GluR2 subunit 
      expression on type-1 and type-2 astrocyte lineage cells. GluR1 subunit expression 
      was more prominent in type-1 than in type-2 astrocytes. To characterize 
      functional properties of glutamate receptors expressed in cultured hippocampal 
      astrocytes, we performed whole-cell patch clamp recording. Application of 
      L-glutamate, AMPA, and kainate, but not NMDA, to small, rounded cells 
      (morphologically identified as type-2 astrocytes) elicited inward currents which 
      were blocked by the AMPA/kainate antagonist 6-cyano-7-nitroquinoxaline-2, 3-dione 
      (CNQX). Cyclothiazide potentiated AMPA- and kainate-elicited currents, indicative 
      of AMPA-preferring receptors. Current voltage analysis indicated that type-2 
      astrocyte AMPA receptors were electrically linear, indicative of 
      GluR2-containing, Ca(2+)-impermeable AMPA receptors. By Northern blot analysis, 
      GluR1 mRNA was highest in astrocyte cultures from cerebellum and hippocampus and 
      moderate in astrocyte cultures from neocortex and striatum. GluR3 mRNA was 
      detectable in astrocyte cultures from cerebellum and neocortex. GluR2 and NR1 
      mRNA expression were not detected in astrocytes cultured from any brain region 
      examined. In situ hybridization studies showed wide expression of GluR1 mRNA in 
      cultured astrocytes; GluR2 and GluR3 mRNAs were near background levels. Thus, 
      cultured type-2 astrocytes express functional AMPA receptors in a cell-specific 
      and region-specific manner, consistent with their role in neuronal-glial 
      communication.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Fan, D
AU  - Fan D
AD  - Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 
      10461, USA.
FAU - Grooms, S Y
AU  - Grooms SY
FAU - Araneda, R C
AU  - Araneda RC
FAU - Johnson, A B
AU  - Johnson AB
FAU - Dobrenis, K
AU  - Dobrenis K
FAU - Kessler, J A
AU  - Kessler JA
FAU - Zukin, R S
AU  - Zukin RS
LA  - eng
GR  - NS 20013/NS/NINDS NIH HHS/United States
GR  - NS 20752/NS/NINDS NIH HHS/United States
GR  - NS 31282/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Receptors, AMPA)
RN  - 77521-29-0 (alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Hippocampus/cytology/*metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Kainic Acid/pharmacology
MH  - Male
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, AMPA/biosynthesis/*physiology
MH  - alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/pharmacology
EDAT- 1999/08/10 00:00
MHDA- 1999/08/10 00:01
CRDT- 1999/08/10 00:00
PHST- 1999/08/10 00:00 [pubmed]
PHST- 1999/08/10 00:01 [medline]
PHST- 1999/08/10 00:00 [entrez]
AID - 10.1002/(SICI)1097-4547(19990815)57:4<557::AID-JNR16>3.0.CO;2-I [pii]
PST - ppublish
SO  - J Neurosci Res. 1999 Aug 15;57(4):557-71.