PMID- 10441368
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20181201
IS  - 8756-7938 (Print)
IS  - 1520-6033 (Linking)
VI  - 15
IP  - 4
DP  - 1999 Jul-Aug
TI  - Production of microspheres with surface amino groups from blends of 
      Poly(Lactide-co-glycolide) and Poly(epsilon-CBZ-L-lysine) and use for 
      encapsulation.
PG  - 763-7
AB  - Microspheres were formed from blends of the biodegradable polymer 
      poly(DL-lactic-co-glycolic acid) (PLGA) together with poly(epsilon-CBZ-L-lysine) 
      (PCBZL) by a double-emulsification/solvent evaporation technique. The size of the 
      microspheres formed by this method was dependent both on the total concentration 
      of the polymers and on the ratio of PLGA to PCBZL. The use of the microspheres 
      for encapsulation was demonstrated by the inclusion of a solution of Texas Red 
      fluorescent dye. Lysine epsilon-amino groups on the surface of the microspheres 
      were deprotected by acid hydrolysis or lithium/liquid ammonia reduction. Acid 
      hydrolysis damaged the surface of the microspheres as assessed by scanning 
      electron microscopy, whereas deprotection by lithium/ammonia produced less damage 
      and allowed the retention of encapsulated dye solution. The surface lysine groups 
      made available on the surface of the microspheres could be used to covalently 
      link a variety of biologically active molecules to alter their in vivo properties 
      and allow targeting to specific cell types.
FAU - Zheng, J
AU  - Zheng J
AD  - Huffington Center on Aging and Department of Cell Biology, Baylor College of 
      Medicine, Houston, Texas 77030, USA.
FAU - Hornsby, P J
AU  - Hornsby PJ
LA  - eng
GR  - AG 12287/AG/NIA NIH HHS/United States
GR  - AG 13663/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biotechnol Prog
JT  - Biotechnology progress
JID - 8506292
RN  - 0 (Biocompatible Materials)
RN  - 0 (Capsules)
RN  - 0 (Emulsions)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Polymers)
RN  - 0 (Solvents)
RN  - 0 (Xanthenes)
RN  - 1155-64-2 (N(epsilon)-carbobenzoxylysine)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 82354-19-6 (Texas red)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Biocompatible Materials/*chemistry
MH  - Capsules
MH  - Emulsions
MH  - Fluorescent Dyes
MH  - Lactic Acid/*chemistry
MH  - Lysine/*analogs & derivatives/chemistry
MH  - Microscopy, Electron, Scanning
MH  - Microspheres
MH  - Particle Size
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry
MH  - Solvents
MH  - Surface Properties
MH  - Xanthenes
EDAT- 1999/08/12 00:00
MHDA- 1999/08/12 00:01
CRDT- 1999/08/12 00:00
PHST- 1999/08/12 00:00 [pubmed]
PHST- 1999/08/12 00:01 [medline]
PHST- 1999/08/12 00:00 [entrez]
AID - bp9900817 [pii]
AID - 10.1021/bp9900817 [doi]
PST - ppublish
SO  - Biotechnol Prog. 1999 Jul-Aug;15(4):763-7. doi: 10.1021/bp9900817.