PMID- 10441379
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20061115
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 368
IP  - 2
DP  - 1999 Aug 15
TI  - Isolation and structural characterization of a cytotoxic L-amino acid oxidase 
      from Agkistrodon contortrix laticinctus snake venom: preliminary crystallographic 
      data.
PG  - 285-90
AB  - We have purified a cytotoxic L-amino acid oxidase (LAO) from Agkistrodon 
      contortrix laticinctus snake venom by means of Superdex-200 gel filtration, 
      followed by phenyl-Sepharose CL-4B chromatography. The purified enzyme (ACL LAO) 
      is a dimer on gel filtration, with a M(r) of 60,000 for the monomer as estimated 
      by SDS-PAGE. LAO activity was tested against 15 amino acids, but only 9 were 
      oxidized by the enzyme, suggesting that it presents some degree of specificity. 
      ACL LAO has apoptosis-inducing activity in an HL-60 cell culture assay. After 24 
      h treatment with 25 micrograms/ml of ACL LAO, the typical DNA fragmentation 
      pattern of apoptotic cells was observed on agarose gel electrophoresis. NMR 
      analysis showed the presence of a flavin mononucleotide prosthetic group. To 
      solve its 3-D structure, crystals of the purified protein were grown in 0.1 M 
      Tris-HCl, pH 8.5, and 2 M (NH(4))(2)SO(4). Diffraction data collected to 3.5 A 
      showed that the protein crystallized in the tetragonal system, with unit cell a = 
      b = 103.22 A, c = 183.45 A. This is the first report of preliminary 
      crystallization data for a snake venom L-amino acid oxidase.
CI  - Copyright 1999 Academic Press.
FAU - Souza, D H
AU  - Souza DH
AD  - Departamento de Ciencias Fisiologicas, Universidade Federal de Sao Carlos, Sao 
      Carlos, SP, 13565-905, Brazil.
FAU - Eugenio, L M
AU  - Eugenio LM
FAU - Fletcher, J E
AU  - Fletcher JE
FAU - Jiang, M S
AU  - Jiang MS
FAU - Garratt, R C
AU  - Garratt RC
FAU - Oliva, G
AU  - Oliva G
FAU - Selistre-de-Araujo, H S
AU  - Selistre-de-Araujo HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Crotalid Venoms)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Agkistrodon
MH  - Amino Acid Oxidoreductases/chemistry/*isolation & purification/toxicity
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Crotalid Venoms/*enzymology
MH  - DNA Fragmentation/drug effects
MH  - HL-60 Cells
MH  - Humans
MH  - L-Amino Acid Oxidase
MH  - Protein Conformation
EDAT- 1999/08/12 00:00
MHDA- 1999/08/12 00:01
CRDT- 1999/08/12 00:00
PHST- 1999/08/12 00:00 [pubmed]
PHST- 1999/08/12 00:01 [medline]
PHST- 1999/08/12 00:00 [entrez]
AID - S0003-9861(99)91287-0 [pii]
AID - 10.1006/abbi.1999.1287 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1999 Aug 15;368(2):285-90. doi: 10.1006/abbi.1999.1287.